Missense mutation of a class B heat shock factor is responsible for the tomato bushy root-2 phenotype
| dc.contributor.author | Kevei, Zoltan | |
| dc.contributor.author | Silva Ferreira, Demetryus | |
| dc.contributor.author | Perez Casenave, Cristina Maria | |
| dc.contributor.author | Kurowski, Tomasz J. | |
| dc.contributor.author | Mohareb, Fady | |
| dc.contributor.author | Rickett, Daniel | |
| dc.contributor.author | Stain, Chris | |
| dc.contributor.author | Thompson, Andrew J. | |
| dc.date.accessioned | 2022-02-09T12:32:34Z | |
| dc.date.available | 2022-02-09T12:32:34Z | |
| dc.date.issued | 2022-02-08 | |
| dc.description.abstract | The bushy root-2 (brt-2) tomato mutant has twisting roots, and slower plant development. Here we used whole genome resequencing and genetic mapping to show that brt-2 is caused by a serine to cysteine (S75C) substitution in the DNA binding domain (DBD) of a heat shock factor class B (HsfB) encoded by SolycHsfB4a. This gene is orthologous to the Arabidopsis SCHIZORIZA gene, also known as AtHsfB4. The brt-2 phenotype is very similar to Arabidopsis lines in which the function of AtHsfB4 is altered: a proliferation of lateral root cap and root meristematic tissues, and a tendency for lateral root cap cells to easily separate. The brt-2 S75C mutation is unusual because all other reported amino acid substitutions in the highly conserved DBD of eukaryotic heat shock factors are dominant negative mutations, but brt-2 is recessive. We further show through reciprocal grafting that brt-2 exerts its effects predominantly through the root genotype even through BRT-2 is expressed at similar levels in both root and shoot meristems. Since AtHsfB4 is induced by root knot nematodes (RKN), and loss-of-function mutants of this gene are resistant to RKNs, BRT-2 could be a target gene for RKN resistance, an important trait in tomato rootstock breeding. | en_UK |
| dc.description.sponsorship | Biotechnology and Biological Sciences Research Council (BBSRC): BB/L01954X/1 | en_UK |
| dc.identifier.citation | Kevei Z, Silva Ferreira DK, Perez Casenave CM, et al., (2022) Missense mutation of a class B heat shock factor is responsible for the tomato bushy root-2 phenotype, Molecular Horticulture, Volume 2, February 2022, Article number 4 | en_UK |
| dc.identifier.issn | 2730-9401 | |
| dc.identifier.uri | https://doi.org/10.1186/s43897-022-00025-0 | |
| dc.identifier.uri | http://dspace.lib.cranfield.ac.uk/handle/1826/17556 | |
| dc.language.iso | en | en_UK |
| dc.publisher | Biomed Central | en_UK |
| dc.rights | Attribution 4.0 International | * |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
| dc.subject | Bushy root-2 | en_UK |
| dc.subject | Genetic mapping | en_UK |
| dc.subject | HsfB4 | en_UK |
| dc.subject | Root knot nematode resistance | en_UK |
| dc.subject | SCHIZORIZA | en_UK |
| dc.subject | Tomato | en_UK |
| dc.title | Missense mutation of a class B heat shock factor is responsible for the tomato bushy root-2 phenotype | en_UK |
| dc.type | Article | en_UK |