Will climate change affect growth and ochratoxin A production of putative biocontrol knockout strains of Aspergillus carbonarius?

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dc.contributor.authorLlobregat, Belén
dc.contributor.authorCervini, Carla
dc.contributor.authorGonzález-Candelas, Luis
dc.contributor.authorVerheecke-Vaessen, Carol
dc.contributor.authorBallester, Ana-Rosa
dc.contributor.authorMedina, Angel
dc.date.accessioned2025-06-02T09:46:35Z
dc.date.available2025-06-02T09:46:35Z
dc.date.freetoread2025-06-02
dc.date.issued2025-08-02
dc.date.pubOnline2025-05-11
dc.description.abstractThe research explored the effects of abiotic factors associated with climate change (CC) on the growth and metabolite production of wild-type Aspergillus carbonarius ITEM 5010 and three knockout mutants: one knockout in the first gene of the ochratoxin A (OTA) biosynthesis pathway (ΔotaA) and two in the veA and laeA genes (the latter knockout generated in this work) encoding VELVET complex proteins, which regulate metabolism. Variables examined were temperature (30 °C vs 37 °C), water activity (0.98 vs 0.90), and CO₂ levels (400 ppm vs 1000 ppm). Growth, OTA production, and other metabolites were evaluated on grape-based medium. The results showed that abiotic factors significantly influenced fungal growth and mycotoxin production, with aw being the most critical parameter. At aw 0.90, no growth was observed. A temperature of 37 °C combined with 1000 ppm CO₂ resulted in higher OTA production, indicating a greater health risk in predicted CC scenarios. Mutants of global regulatory factors showed altered metabolite production, with elevated OTA levels at 37 °C. The ΔotaA knockout mutant consistently showed no OTA production, suggesting its viability as a biocontrol agent under CC conditions. However, while OTA increased, other secondary metabolites, such as pyranonigrin A and kojic acid, decreased with rising temperatures in all strains. The research highlights the influence of abiotic factors related to CC on A. carbonarius growth and metabolite production, underlining the threat of increased mycotoxin production. This reinforces the need for resilient biocontrol strategies. The ΔotaA mutant has been identified as a potential biocontrol agent, demonstrating resistance to future environmental stresses associated with CC.
dc.description.journalNameInternational Journal of Food Microbiology
dc.description.sponsorshipMinisterio de Ciencia, Innovación y Universidades; AGROALNEXT/2022/028
dc.description.sponsorshipThis work was partially funded by projects PID2021-126005OBI00 (MICIU/AEI/10.13039/501100011033/FEDER, UE), AGROALNEXT/2022/028 (GVA, PRTR, MRR, NextGenerationEU), and PIE 202270I070 (CSIC).
dc.format.mediumPrint-Electronic
dc.identifier.citationLlobregat B, Cervini C, González-Candelas L, et al., (2025) Will climate change affect growth and ochratoxin A production of putative biocontrol knockout strains of Aspergillus carbonarius?. International Journal of Food Microbiology, Volume 439, August 2025, Article number 111250en_UK
dc.identifier.eissn1879-3460
dc.identifier.elementsID673141
dc.identifier.issn0168-1605
dc.identifier.paperNo111250
dc.identifier.urihttps://doi.org/10.1016/j.ijfoodmicro.2025.111250
dc.identifier.urihttps://dspace.lib.cranfield.ac.uk/handle/1826/23950
dc.identifier.volumeNo439
dc.languageEnglish
dc.language.isoen
dc.publisherElsevieren_UK
dc.publisher.urihttps://www.sciencedirect.com/science/article/pii/S0168160525001953?via%3Dihub
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAspergillus carbonariusen_UK
dc.subjectBiocontrol agenten_UK
dc.subjectClimate changeen_UK
dc.subjectKojic aciden_UK
dc.subjectOchratoxin Aen_UK
dc.subjectPyranonigrin Aen_UK
dc.subject3108 Plant Biologyen_UK
dc.subject31 Biological Sciencesen_UK
dc.subjectInfectious Diseasesen_UK
dc.subjectGeneticsen_UK
dc.subjectMicrobiologyen_UK
dc.subject3006 Food sciencesen_UK
dc.titleWill climate change affect growth and ochratoxin A production of putative biocontrol knockout strains of Aspergillus carbonarius?en_UK
dc.typeArticle
dc.type.subtypeJournal Article
dcterms.dateAccepted2025-05-07

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