In vitro assay and inhibition of 9-cis-epoxycarotenoid dioxygenase (NCED) from Solanum lycopersicum and Zea mays

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dc.contributor.authorHarrison, Peter J.
dc.contributor.authorChandler, Jake
dc.contributor.authorThompson, Andrew J.
dc.contributor.authorBugg, Timothy D. H.
dc.date.accessioned2024-08-13T10:31:45Z
dc.date.available2024-08-13T10:31:45Z
dc.date.freetoread2024-10-07
dc.date.issued2024
dc.date.pubOnline2024-06-06
dc.description.abstractThe article reports methods for the expression and assay of 9-cis-epoxycarotenoid cleavage dioxygenase (NCED), an enzyme involved in the biosynthesis of phytohormone abscisic acid in plants. A method for the preparation of the unstable substrate 9′-cis-neoxanthin from fresh spinach is described. The inhibition of Solanum lycopersicum NCED by a series of aryl hydroxamic acid inhibitors is illustrated, and inhibitors D2 and D4 are assayed against NCED isozymes from Zea mays.
dc.description.bookTitleMethods in Enzymeology
dc.description.sponsorshipBiotechnology and Biological Sciences Research Council (BBSRC)
dc.description.sponsorshipThis work was initiated under BBSRC SCIBS project BB/D005787/1 awarded to AJT and THDB.
dc.identifier.citationHarrison PJ, Chandler J, Thompson AJ, Bugg TD. (2024) In vitro assay and inhibition of 9-cis-epoxycarotenoid dioxygenase (NCED) from Solanum lycopersicum and Zea mays. Methods in Enzymology (Book Series: Mononuclear Non-heme Iron Dependent Enzymes Part B), Volume 704, September 2024, pp. 291-312
dc.identifier.eissn1557-7988
dc.identifier.issn0076-6879
dc.identifier.urihttps://doi.org/10.1016/bs.mie.2024.05.012
dc.identifier.urihttps://dspace.lib.cranfield.ac.uk/handle/1826/22777
dc.language.isoen
dc.publisherElsevier
dc.publisher.urihttps://doi.org/10.1016/bs.mie.2024.05.012
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titleIn vitro assay and inhibition of 9-cis-epoxycarotenoid dioxygenase (NCED) from Solanum lycopersicum and Zea mays
dc.typeBook chapter
dcterms.dateAccepted2024

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