In vitro assay and inhibition of 9-cis-epoxycarotenoid dioxygenase (NCED) from Solanum lycopersicum and Zea mays
Date published
2024
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2024-10-07
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Elsevier
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Book chapter
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0076-6879
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Harrison PJ, Chandler J, Thompson AJ, Bugg TD. (2024) In vitro assay and inhibition of 9-cis-epoxycarotenoid dioxygenase (NCED) from Solanum lycopersicum and Zea mays. Methods in Enzymology (Book Series: Mononuclear Non-heme Iron Dependent Enzymes Part B), Volume 704, September 2024, pp. 291-312
Abstract
The article reports methods for the expression and assay of 9-cis-epoxycarotenoid cleavage dioxygenase (NCED), an enzyme involved in the biosynthesis of phytohormone abscisic acid in plants. A method for the preparation of the unstable substrate 9′-cis-neoxanthin from fresh spinach is described. The inhibition of Solanum lycopersicum NCED by a series of aryl hydroxamic acid inhibitors is illustrated, and inhibitors D2 and D4 are assayed against NCED isozymes from Zea mays.
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Attribution-NonCommercial-NoDerivatives 4.0 International
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Biotechnology and Biological Sciences Research Council (BBSRC)
This work was initiated under BBSRC SCIBS project BB/D005787/1 awarded to AJT and THDB.
This work was initiated under BBSRC SCIBS project BB/D005787/1 awarded to AJT and THDB.