A novel enzyme entrapment in SU-8 microfabricated films for glucose micro-biosensors
| dc.contributor.author | Psoma, Sotiria D. | |
| dc.contributor.author | van der Wal, Peter D. | |
| dc.contributor.author | Frey, Olivier | |
| dc.contributor.author | de Rooij, Nicolaas F. | |
| dc.contributor.author | Turner, Anthony P. F. | |
| dc.date.accessioned | 2011-01-24T15:23:36Z | |
| dc.date.available | 2011-01-24T15:23:36Z | |
| dc.date.issued | 2010-12-15 | |
| dc.description.abstract | The present work investigates the utilisation of the widely used SU-8 photoresist as an immobilisation matrix for glucose oxidase (GOx) for the development of glucose micro-biosensors. The strong advantage of the proposed approach is the simultaneous enzyme entrapment during the microfabrication process within a single step, which is of high importance for the simplification of the BioMEMS procedures. Successful encapsulation of the enzyme GOx in “customised” SU-8 microfabricated structures was achieved through optimisation of the one-step microfabrication process. Although the process involved contact with organic solvents, UV-light exposure, heating for pre- and post-bake and enzyme entrapment in a hard and rigid epoxy resin matrix, the enzyme retained its activity after encapsulation in SU-8. Measurements of the immobilised enzyme's activity inside the SU-8 matrix were carried out using amperometric detection of hydrogen peroxide in a 3-electrode setup. Films without enzyme showed negligible variation in current upon the addition of glucose, as opposed to films with encapsulated enzyme which showed a very clear increase in current. Experiments using films of increased thickness or enzyme concentration, showed a higher response, thus proving that the enzyme remained active not only on the film's surface, but also inside the matrix as well. The proposed enzyme immobilisation in SU-8 films opens up new possibilities for combining BioMEMS with biosensors and organic electronics. | en_UK |
| dc.identifier.citation | Sotiria D. Psoma, Peter D. van der Wal, Olivier Frey, Nicolaas F. de Rooij and Anthony P.F. Turner, A novel enzyme entrapment in SU-8 microfabricated films for glucose micro-biosensors, Biosensors and Bioelectronics, Volume 26, Issue 4, 15 December 2010, Pages 1582-1587 | en_UK |
| dc.identifier.issn | 0956-5663 | |
| dc.identifier.uri | http://dx.doi.org/10.1016/j.bios.2010.07.117 | |
| dc.identifier.uri | http://dspace.lib.cranfield.ac.uk/handle/1826/4761 | |
| dc.language.iso | en | en_UK |
| dc.publisher | Elsevier | en_UK |
| dc.subject | SU-8 films | en_UK |
| dc.subject | Biosensor | en_UK |
| dc.subject | Microfabrication | en_UK |
| dc.subject | Immobilisation | en_UK |
| dc.subject | Glucose oxidase entrapment | en_UK |
| dc.subject | BioMEMS | en_UK |
| dc.title | A novel enzyme entrapment in SU-8 microfabricated films for glucose micro-biosensors | en_UK |
| dc.type | Article | en_UK |