A novel enzyme entrapment in SU-8 microfabricated films for glucose micro-biosensors

Date published

2010-12-15

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Elsevier

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Article

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0956-5663

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Sotiria D. Psoma, Peter D. van der Wal, Olivier Frey, Nicolaas F. de Rooij and Anthony P.F. Turner, A novel enzyme entrapment in SU-8 microfabricated films for glucose micro-biosensors, Biosensors and Bioelectronics, Volume 26, Issue 4, 15 December 2010, Pages 1582-1587

Abstract

The present work investigates the utilisation of the widely used SU-8 photoresist as an immobilisation matrix for glucose oxidase (GOx) for the development of glucose micro-biosensors. The strong advantage of the proposed approach is the simultaneous enzyme entrapment during the microfabrication process within a single step, which is of high importance for the simplification of the BioMEMS procedures. Successful encapsulation of the enzyme GOx in “customised” SU-8 microfabricated structures was achieved through optimisation of the one-step microfabrication process. Although the process involved contact with organic solvents, UV-light exposure, heating for pre- and post-bake and enzyme entrapment in a hard and rigid epoxy resin matrix, the enzyme retained its activity after encapsulation in SU-8. Measurements of the immobilised enzyme's activity inside the SU-8 matrix were carried out using amperometric detection of hydrogen peroxide in a 3-electrode setup. Films without enzyme showed negligible variation in current upon the addition of glucose, as opposed to films with encapsulated enzyme which showed a very clear increase in current. Experiments using films of increased thickness or enzyme concentration, showed a higher response, thus proving that the enzyme remained active not only on the film's surface, but also inside the matrix as well. The proposed enzyme immobilisation in SU-8 films opens up new possibilities for combining BioMEMS with biosensors and organic electronics.

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Keywords

SU-8 films, Biosensor, Microfabrication, Immobilisation, Glucose oxidase entrapment, BioMEMS

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