Analysis of MRSA Staphylococcal Chromosome cassette mecA status from next generation sequence data
Date
2012-03
Authors
Supervisor/s
Journal Title
Journal ISSN
Volume Title
Publisher
Cranfield University
Department
SAS
Type
Thesis or dissertation
ISSN
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Citation
Abstract
NGS sequencing libraries prepared on an Illumina NGS platform for 10 isolates of Staphylococcus aureus were analysed. After extensive pre-processing to address library quality issues, for each isolate the status of the Staphylococcal Chromosome Cassette, and its mecA gene specifying resistance to meticillin, was determined. All mecA-positive isolates encoded canonical mecA. None encoded the new variant mecA identified in strain LGA251.
Description
Software Description
Software Language
Github
Keywords
core genome, accessory genome, NGS sequencing libraries, drug resistance