Browsing by Author "Levina, Jan-Olof"
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Item Open Access Amperometric biosensor for formic acid in air(Elsevier, 2000-11-01) Sandström, K. J. Mattias; Newman, Jeffrey D.; Sunesson, Anna-Lena; Levina, Jan-Olof; Turner, Anthony P. F.The possibility of developing a simple, inexpensive and specific personal passive ”real-time” air sampler based on biosensor technology was investigated. Formic acid was used as a model substance. The sensor is based on the enzymatic reaction between formic acid and formate dehydrogenase with NAD+ as a cofactor and Meldola’s blue as mediator. An effective way to immobilise the enzyme, cofactor and Meldola’s blue on screen-printed electrodes was found to be in a mixture of glycerol and phosphate buffer covered with a gas-permeable membrane. When the sensor was introduced into an atmosphere containing formic acid, it gave a distinct and rapid amperometric response.