Browsing by Author "Collings, Emma"
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Item Open Access Data for the paper: Spatial changes in leaf biochemical profile of two tea cultivars following cold storage under two different vapour pressure deficit (VPD) conditions(Cranfield University, 2018-10-24 10:33) del carmen Alamar Gavidia, Maria; Collings, Emma; Terry, LeonUnderlying data for tea spatial paper which includes: catechin, moisture, respiration, ethylene and colourItem Open Access Data underpinning "Seasonal and Temporal Changes during Storage Affect Quality Attributes of Green Asparagus"(Cranfield University, 2019-09-19 18:33) Anastasiadi, Maria; Collings, Emma; Terry, Leon; Shivembe, Allan; Qian, BinghuaThis dataset contains the data used for statistical analysis and predictive modelling in the paper entitled "Seasonal and Temporal Changes during Storage Affect Quality Attributes of Green Asparagus". Specifically it contains physiological and biochemical changes in asparagus spears from two different cultivars during shelf-life captured over the course of the harvest season, as well as during cold storage and subsequent shelf-life for three different cultivars. Physiological changes include moisture loss, respiration rate, cutting energy, stiffness, objective colour. Biochemical data include individual sugars, ascorbic acid and abscisic acid and its catabolites. Also the data capture spatial differences along the asparagus spears (apical and basal regions).Item Open Access Discrete ex situ and continuous in situ real-time respiration rate measurements of fresh produce using a novel automated dynamic approach(International Society for Horticultural Science (ISHS), 2018-04-04) Collings, Emma; Alamar, M. Carmen; Cools, Katherine; Ordaz-Ortiz, J. J.; Terry, Leon A.Fresh produce respiration rate is a useful indicator of metabolic state often used in postharvest research to determine physiological differences between factors. Static (no flow) and dynamic (constant flow) measurements are two types of methods that exist for determining respiration rate in fresh produce. Implementation of an automated real-time respiration method, using a dynamic measurement, for measuring respiration rates of fresh produce in discrete (ex situ) and continuous (in situ) set-ups has been established. Discrete methods were performed on fresh (green) black pepper within 3 L hermetically sealed containers with constant flow rates of 400 mL min-1 and 1 L min-1, respectively. Continuous respiration measurements were also obtained in situ for fresh black pepper stored under air (1.8 L min-1) at 5°C, and avocado fruit stored under air and controlled atmosphere environment (ca. 10 kPa CO2 and 10 kPa O2; 400 mL min-1), at 20°C. In addition, simultaneous automated recordings of O2 and CO2 enabled accurate respiratory quotient (RQ) values to be determined for avocado throughout storage. Application of the continuous dynamic in situ measurements can also provide a more realistic assessment of physiological change / behaviour under real-world storage conditions. In this paper, we discuss the application of both discrete and continuous dynamic methods as powerful research techniques for measuring respiration rate in postharvest research.Item Open Access Improving the tea withering process using ethylene or UV-C(Cranfield University, 2021-12-13 11:24) del carmen Alamar Gavidia, Maria; Terry, Leon; Collings, Emma; Thompson, Andrew; Mohareb, Fady; Kourmpetli, Sofia; Kevei, Zoltan; Bogaerts Marquez, MariaThe data set contains objective colour, respiration rate, water loss data, as well as individual catechin and theobromine concentrations of Camellia sinensis shoots subjected to UV-C radiation and ethylene supplementation during simulated withering.Item Open Access Underpinning data 'Investigating the role of abscisic acid and its catabolites on senescence processes in green asparagus under controlled atmosphere (CA) storage regimes'(Cranfield University, 2022-03-25 10:47) Anastasiadi, Maria; Collings, Emma; Terry, LeonThis dataset contains the data used for statistical analysis and predictive modelling in the paper entitled "Revisiting CA Protocols and Their Effect on Hormonal Flux in Asparagus". Specifically it contains physiological and biochemical changes in asparagus spears from three different cultivars during cold storage under different CA and DCA regimes and subsequent shelf-life. Physiological changes include moisture loss, respiration rate, cutting energy, objective colour. Biochemical data include individual sugars, abscisic acid and its catabolites. Also the data capture spatial differences along the asparagus spears (apical and basal regions).Item Open Access Understanding asparagus tip breakdown postharvest at a molecular and cellular level(International Society for Horticultural Science, 2023-10-09) Nayakoti, Swapna; Collings, Emma; Landahl, Sandra; Chinn, John; Terry, Leon A.; Christofides, S.; Alamer, M. Carmen; Rogers, HilaryAsparagus has a very limited season in the UK and a short shelf-life. Tip breakdown occurs sporadically during chilled storage and limits shelf-life. It is characterized by internal browning and is accompanied by an unpleasant aroma. The change in aroma can be the first sign of the breakdown, and results in waste as whole batches of asparagus need to be discarded. Data suggest that the breakdown may not be initiated by microbial action, but the underlying mechanisms are yet to be fully elucidated. Tip breakdown occurs more frequently in faster growing spears when growing temperatures are higher. To explore whether the faster growth is associated with larger cells, cell size was compared in spear tips of a cultivar which is more susceptible to tip rot, ‘Aspalim’, and a cultivar in which tip rot very rarely occurs, ‘Early California’. However, no significant differences were found. Transcriptomes were sequenced from healthy spears, and spears in which symptoms of tip breakdown could be seen visually, to assess changes in gene expression during the development of the tip breakdown. More genes were upregulated in spears showing tip breakdown compared to healthy spears after 14 days of chilled storage. The largest functional group of differentially expressed genes is related to DNA binding and transcription regulation which may provide useful information on the overall regulation of the breakdown process.Item Embargo Understanding the mechanisms responsible for postharvest tip breakdown in asparagus: a biochemical approach(International Society for Horticultural Science, 2023-10-09) Collings, Emma; Landahl, Sandra; Jodkowska, Monika; Nayakoti, Swapna; Chinn, John; Rogers, Hilary; Terry, Leon A.; Alamar, M. CarmenThe UK asparagus season is relatively short (from April to June), resulting in large quantities of spears being imported to provide year-round supply. Currently, asparagus quality can only be maintained for one week, plus seven days shelf-life, using cold storage alone. A limiting factor, which contributes significantly towards commercial losses, is the development of the postharvest physiological disorder tip breakdown (also known as tip rot). The disorder is characterised by darkening tips followed by the presence of water-soaked bracts at the tip of the spear and a foul odour. However, the underlying causes of tip breakdown have not yet been fully elucidated. We hypothesise that fast growth, slow rate of sugar transport and localised cell death may be key components of the mechanism. To gain more insight into tip breakdown development, two asparagus cultivars with low and high susceptibility to tip breakdown (‘Early California’ and ‘Aspalim’, respectively) were grown under controlled conditions and subjected to warm (28/14 °C) and cold (18/8 °C) day/ night temperatures. Harvested spears were subjected to shelf-life storage, 14 days at 7 °C, to subjectively assess early symptoms and further development of tip breakdown. Spears were snap frozen for subsequent biochemical analysis (viz. non-structural carbohydrates and plant growth regulators [PGRs]). Results showed that ‘Aspalim’ spears grown at warmer temperatures had higher incidence of tip breakdown than ‘Early California’. The relationship between incidence and severity of asparagus tip breakdown and the role of PGRs and sugar content during the season, as affected by genotype and growing conditions, are also discussed. The fundamental mechanistic understanding of asparagus tip breakdown will aid to implement optimum postharvest storage strategies to reduce food loss and the associated carbon footprint.