A genetic approach to improvements of Candida tropicalis as a biocatalyst

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dc.contributor.advisorWarner, P. J.
dc.contributor.authorPerry, Patricia Mair
dc.date.accessioned2016-03-11T15:49:58Z
dc.date.available2016-03-11T15:49:58Z
dc.date.issued1990-11
dc.description.abstractIt is well documented in the literature that, in addition to carbon sources such as glucose, Candida species are able to utilise a wide variety of n-alkanes which are degraded via beta-oxidation. Prior to beta-oxidation a primary oxidation system acts on the end terminal methyl groups to generate carboxyl groups. The main industrial aim of the work was to optimise the bioconversion of pelargonate [CH3(CH2)7COOH] to azeleate [HOOC(CH2)7COOH], i.e. the primary oxidation steps, by blocking the beta-oxidation pathway in C. tropicalis. To this end, a library of Sau 3AI partially digested C. tropicalis NCYC997 genomic DNA in pBR322 was constructed from which it was hoped to isolate and disrupt the long-chain fatty acid acyl-CoA oxidase genes, POX4 and POX5, which catalyse the first step of beta-oxidation. The library was probed with oligonucleotides specific to POX4 and POX5, and a putative POX4 clone was isolated.en_UK
dc.description.sponsorshipChemGen Corporationen_UK
dc.identifier.citationPerry, P.M. 1990. A genetic approach to improvements of Candida tropicalis as a biocatalyst. Cranfield University (PhD thesis)en_UK
dc.identifier.urihttp://dspace.lib.cranfield.ac.uk/handle/1826/9784
dc.language.isoenen_UK
dc.publisherCranfield Universityen_UK
dc.publisher.departmentCranfield Institute of Technology. Biotechnology Centreen_UK
dc.titleA genetic approach to improvements of Candida tropicalis as a biocatalysten_UK
dc.typeThesis or dissertationen_UK
dc.type.qualificationlevelDoctoralen_UK
dc.type.qualificationnamePhDen_UK

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