Unignorable enzyme-specific isotope fractionation for nitrate source identification in aquatic ecosystem

Simple item page
dc.contributor.authorWang, Shuo
dc.contributor.authorLyu, Tao
dc.contributor.authorLi, Shengjie
dc.contributor.authorJiang, Zhuo
dc.contributor.authorDang, Zhengzhu
dc.contributor.authorZhu, Xianfang
dc.contributor.authorHu, Wei
dc.contributor.authorYue, Fu-jun
dc.contributor.authorJi, Guodong
dc.date.accessioned2023-11-22T16:37:27Z
dc.date.available2023-11-22T16:37:27Z
dc.date.issued2023-11-22
dc.description.abstractNitrate contamination in aquatic systems is a widespread problem across the world. The isotopic composition (δ15N, δ18O) of nitrate and their isotope effect (15ε, 18ε) can facilitate the identification of the source and transformation of nitrate. Although previous researches claimed the isotope fractionations may change the original δ15N/δ18O values and further bias identification of nitrate sources, isotope effect was often ignored due to its complexity. To fill the gap between the understanding and application, it is crucial to develop a deep understanding of isotopic fractionation based on available evidence. In this regard, this study summarized the available methods to determine isotope effects, thereby systematically comparing the magnitude of isotope effects (15ε and 18ε) in nitrification, denitrification and anammox. We found that the enzymatic reaction plays the key role in isotope fractionations, which is significantly affected by the difference in the affinity, substrate channel properties and redox potential of active site. Due to the overlapping of microbial processes and accumulation of uncertainties, the significant isotope effects at small scales inevitably decrease in large-scale ecosystems. However, the proportionality of N and O isotope fractionation (δ18O/δ15N; 18ε/15ε) associated with nitrate reduction generally follows enzyme-specific proportionalities (i.e., Nar, 0.95; Nap, 0.57; eukNR, 0.98) in aquatic ecosystems, providing enzyme-specific constant factors for the identification of nitrate transformation. With these results, this study finally discussed feasible source portioning methods when considering the isotope effect and aimed to improve the accuracy in nitrate source identification.en_UK
dc.identifier.citationWang S, Lyu T, Li S, et al., (2024) Unignorable enzyme-specific isotope fractionation for nitrate source identification in aquatic ecosystem. Chemosphere, Volume 248, January 2024, Article number 140771en_UK
dc.identifier.issn0045-6535
dc.identifier.urihttps://doi.org/10.1016/j.chemosphere.2023.140771
dc.identifier.urihttps://dspace.lib.cranfield.ac.uk/handle/1826/20576
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectEutrophication controlen_UK
dc.subjectNitrate source identificationen_UK
dc.subjectEnzyme-specific isotope effectsen_UK
dc.subjectStable isotopesen_UK
dc.titleUnignorable enzyme-specific isotope fractionation for nitrate source identification in aquatic ecosystemen_UK
dc.typeArticleen_UK

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Unignorable_enzyme-specific_isotope_fractionation-2023.pdf
Size:
2.36 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.63 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Staff publications (SWEE)