The influence of ecophysiological factors on growth, aflR gene expression and aflatoxin B1 production by a type strain of Aspergillus flavus

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dc.contributor.authorBernaldez, Victoria
dc.contributor.authorCordoba, Juan J.
dc.contributor.authorMagan, Naresh
dc.contributor.authorPeromingo, Belén
dc.contributor.authorRodriguez, Alicia
dc.date.accessioned2017-06-09T11:46:04Z
dc.date.available2017-06-09T11:46:04Z
dc.date.issued2017-05-18
dc.description.abstractMaize is prone to infection by Aspergillus flavus, which can contaminate the product with aflatoxins. The objective of this study was to examine the impact that interactions between water activity (aw) and temperature may have on growth, the expression of a biosynthetic regulatory gene (aflR) and aflatoxin B1 (AFB1) production by a strain of A. flavus on a maize-based medium. Results showed that there were some differences between lag phases and growth rates of A. flavus. The optimum growth rate for A. flavus was at 30 °C and 0.99 aw. No growth occurred at 0.90 aw and 20 °C. Both temperature and aw had an influence on the relative aflR gene expression and AFB1 production by A. flavus; however, the results for AFB1 production were not consistent with the effects on gene expression or growth. These findings provide data that are useful to better understand the conditions which represent higher risks from AFB1 production. However, the aflR expression was not a good indicator of AFB1 production alone. Thus, further molecular studies of other AF-related genes should be done. These results are discussed in the context of harvesting and storage of maize and the prevailing environmental conditions to minimise AFB1 contamination.en_UK
dc.identifier.citationVictoria Bernáldez, Juan J. Córdoba, Naresh Magan, Belén Peromingo, Alicia Rodríguez, The influence of ecophysiological factors on growth, aflR gene expression and aflatoxin B1 production by a type strain of Aspergillus flavus, LWT - Food Science and Technology, Volume 83, 15 September 2017, Pages 283-291en_UK
dc.identifier.issn0023-6438
dc.identifier.urihttp://dxdoi.org/10.1016/j.lwt.2017.05.030
dc.identifier.urihttp://dspace.lib.cranfield.ac.uk/handle/1826/11990
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectMaizeen_UK
dc.subjectAflatoxinen_UK
dc.subjectAspergillus flavusen_UK
dc.subjectaflRen_UK
dc.subjectEnvironmenten_UK
dc.titleThe influence of ecophysiological factors on growth, aflR gene expression and aflatoxin B1 production by a type strain of Aspergillus flavusen_UK
dc.typeArticleen_UK

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