Effects of mutation of 2,3-butanediol formation pathway on glycerol metabolism and 1,3-propanediol production by Klebsiella pneumoniae J2B

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dc.contributor.authorKumar, Vinod
dc.contributor.authorDurgapal, Meetu
dc.contributor.authorSankaranarayanan, Mugesh
dc.contributor.authorSomasundar, Ashok
dc.contributor.authorRathnasingh, Chelladurai
dc.contributor.authorSong, HyoHak
dc.contributor.authorSeung, Doyoung
dc.contributor.authorPark, Sunghoon
dc.date.accessioned2017-04-21T07:53:32Z
dc.date.available2017-04-21T07:53:32Z
dc.date.issued2016-04-22
dc.description.abstractThe current study investigates the impact of mutation of 2,3-butanediol (BDO) formation pathway on glycerol metabolism and 1,3-propanediol (PDO) production by lactate dehydrogenase deficient mutant of Klebsiella pneumoniae J2B. To this end, BDO pathway genes, budA, budB, budC and budO (whole-bud operon), were deleted from K. pneumoniae J2B ΔldhA and the mutants were studied for glycerol metabolism and alcohols (PDO, BDO) production. ΔbudO-mutant-only could completely abolish BDO production, but with reductions in cell growth and PDO production. By modifying the culture medium, the ΔbudO mutant could recover its performance on the flask scale. However, in bioreactor experiments, the ΔbudO mutant accumulated a significant amount of pyruvate (>73 mM) in the late phase and PDO production stopped concomitantly. Glycolytic intermediates of glycerol, especially glyceraldehyde-3-phosphate (G3P) was highly inhibitory to glycerol dehydratase (GDHt); its accumulation, followed by pyruvate accumulation, was assumed to be responsible for the ΔbudO mutant’s low PDO production.en_UK
dc.identifier.citationVinod Kumar, Meetu Durgapal, Mugesh Sankaranarayanan, Ashok Somasundar, Chelladurai Rathnasingh, HyoHak Song, Doyoung Seung, Sunghoon Park, Effects of mutation of 2,3-butanediol formation pathway on glycerol metabolism and 1,3-propanediol production by Klebsiella pneumoniae J2B, Bioresource Technology, Volume 214, August 2016, Pages 432-440en_UK
dc.identifier.cris16221024
dc.identifier.issn0960-8524
dc.identifier.urihttp://dx.doi.org/10.1016/j.biortech.2016.04.032
dc.identifier.urihttp://dspace.lib.cranfield.ac.uk/handle/1826/11796
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectKlebsiella pneumoniae J2Ben_UK
dc.subjectGlycerolen_UK
dc.subject1,3-Propanediolen_UK
dc.subject2,3-Butanediolen_UK
dc.subjectΔbudO mutanten_UK
dc.titleEffects of mutation of 2,3-butanediol formation pathway on glycerol metabolism and 1,3-propanediol production by Klebsiella pneumoniae J2Ben_UK
dc.typeArticleen_UK

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