Selection of imprinted nanoparticles by affinity chromatography
Date
2009-04-15T00:00:00Z
Supervisor/s
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier Science B.V., Amsterdam.
Department
Type
Article
ISSN
0956-5663
Format
Citation
Guerreiro AR, Chianella I, Piletska E, Whitcombe MJ, Piletsky SA. (2009) Selection of imprinted nanoparticles by affinity chromatography, Biosensors and Bioelectronics, Volume 24, Issue 8, April 2009, pp. 2740-2743
Abstract
Soluble molecularly imprinted nanoparticles were synthesised via iniferter initiated polymerisation and separated by size via gel permeation chromatography. Subsequent fractionation of these particles by affinity chromatography allowed the separation of high affinity fractions from the mixture of nanoparticles. Fractions selected this way possess affinity similar to that of natural antibodies (Kd 6.6 × 10−8) M and were also able to discriminate between related functional analogues of the template.
Description
Software Description
Software Language
Github
Keywords
Molecularly imprinted polymer, Nanoparticle, Affinity chromatography