Flavivirus integrations in Aedes aegypti are limited and highly conserved across samples from different geographic regions unlike integrations in Aedes albopictus

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dc.contributor.authorSpadar, Anton
dc.contributor.authorPhelan, Jody
dc.contributor.authorBenavente, Ernest Diez
dc.contributor.authorCampos, Monica
dc.contributor.authorGomez, Lara Ferrero
dc.contributor.authorMohareb, Fady
dc.contributor.authorClark, Taane G.
dc.contributor.authorCampino, Susana
dc.date.accessioned2021-07-08T13:29:11Z
dc.date.available2021-07-08T13:29:11Z
dc.date.issued2021-06-26
dc.description.abstractMosquitoes of the genus Aedes are the main vectors of many viruses, e.g. dengue and Zika, which affect millions of people each year and for which there are limited treatment options. Understanding how Aedes mosquitoes tolerate high viral loads may lead to better disease control strategies. Elucidating endogenous viral elements (EVEs) within vector genomes may give exploitable biological insights. Previous studies have reported the presence of a large number of EVEs in Aedes genomes. Here we investigated if flavivirus EVEs are conserved across populations and different Aedes species by using ~ 500 whole genome sequence libraries from Aedes aegypti and Aedes albopictus, sourced from colonies and field mosquitoes across continents. We found that nearly all flavivirus EVEs in the Ae. aegypti reference genome originate from four separate putative viral integration events, and that they are highly conserved across geographically diverse samples. By contrast, flavivirus EVEs in the Ae. albopictus reference genome originate from up to nine distinct integration events and show low levels of conservation, even within samples from narrow geographical ranges. Our analysis suggests that flaviviruses integrated as long sequences and were subsequently fragmented and shuffled by transposable elements. Given that EVEs of Ae. aegypti and Ae. albopictus belong to different phylogenetic clades and have very differing levels of conservation, they may have different evolutionary origins and potentially different functional roles.en_UK
dc.identifier.citationSpadar A, Phelan JE, Benavente ED, et al., (2021) Flavivirus integrations in Aedes aegypti are limited and highly conserved across samples from different geographic regions unlike integrations in Aedes albopictus. Parasites and Vectors, Volume 14, 2021, Article number 332en_UK
dc.identifier.issn1756-3305
dc.identifier.urihttps://doi.org/10.1186/s13071-021-04828-w
dc.identifier.urihttp://dspace.lib.cranfield.ac.uk/handle/1826/16859
dc.language.isoenen_UK
dc.publisherSpringeren_UK
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectEndogenous viral elementen_UK
dc.subjectArbovirusen_UK
dc.subjectFlavivirusen_UK
dc.subjectAedesen_UK
dc.subjectMosquitoen_UK
dc.titleFlavivirus integrations in Aedes aegypti are limited and highly conserved across samples from different geographic regions unlike integrations in Aedes albopictusen_UK
dc.typeArticleen_UK

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