Labeless AC impedimetric antibody-based sensors with pg ml-1 sensitivities for point-of-care biomedical applications.

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dc.contributor.authorBarton, Andrew C.-
dc.contributor.authorCollyer, Stuart D.-
dc.contributor.authorDavis, Frank-
dc.contributor.authorGarifallou, Goulielmos-Zois-
dc.contributor.authorTsekenis, Georgios-
dc.contributor.authorTully, Elizabeth-
dc.contributor.authorO'Kennedy, Richard-
dc.contributor.authorGibson, Tim-
dc.contributor.authorMillner, Paul A.-
dc.contributor.authorHigson, Seamus P. J.-
dc.date.accessioned2011-10-11T07:32:55Z
dc.date.available2011-10-11T07:32:55Z
dc.date.issued2009-01-01T00:00:00Z-
dc.description.abstractThis paper describes the development and characterisation of labeless immunosensors for (a) the cardiac drug digoxin and (b) bovine serum albumin (BSA). Commercial screen-printed carbon electrodes were used as the basis for the sensors. Two methods were used to immobilise antibodies at the electrode surface. Aniline was electropolymerised onto these electrodes to form a thin planar film of conductive polyaniline; the polyaniline film was then utilised as a substrate to immobilise biotinylated anti-digoxin using a classical avidin- biotin affinity approach. As an alternative approach, poly(1,2-diaminobenzene) was electrodeposited onto the carbon electrodes and this modified surface was then sonochemically ablated to form an array of micropores. A second electropolymerisation step was then used to co-deposit conductive polyaniline along with antibodies for BSA within these pores to produce a microarray of polyaniline protrusions with diameters of several μm, containing entrapped anti- BSAen_UK
dc.identifier.citationAndrew C. Barton, Stuart D. Collyer, Frank Davis, Goulielmos-Zois Garifallou, Georgios Tsekenis, Elizabeth Tully, Richard O'Kennedy, Tim Gibson, Paul A. Millner, Seamus P.J. Higson, Labeless AC impedimetric antibody-based sensors with pg ml-1 sensitivities for point-of-care biomedical applications, Biosensors and Bioelectronics, Volume 24, Issue 5, Selected Papers from the Tenth World Congress on Biosensors Shangai, China, May 14-16, 2008, 1 January 2009, Pages 1090-1095-
dc.identifier.issn0956-5663-
dc.identifier.urihttp://dx.doi.org/10.1016/j.bios.2008.06.001-
dc.identifier.urihttp://dspace.lib.cranfield.ac.uk/handle/1826/3196
dc.language.isoen_UK-
dc.publisherElsevier Science B.V., Amsterdam.en_UK
dc.subjectAC impedanceen_UK
dc.subjectImmunosensoren_UK
dc.subjectPolyanilineen_UK
dc.subjectMicroelectrodeen_UK
dc.subjectBSAen_UK
dc.subjectDigoxinen_UK
dc.titleLabeless AC impedimetric antibody-based sensors with pg ml-1 sensitivities for point-of-care biomedical applications.en_UK
dc.typeArticle-

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