Biomass extraction using non-chlorinated solvents for biocompatibility improvement of polyhydroxyalkanoates

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dc.contributor.authorJiang, Guozhan
dc.contributor.authorJohnston, Brian
dc.contributor.authorTownrow, David E.
dc.contributor.authorRadecka, Iza
dc.contributor.authorKoller, Martin
dc.contributor.authorChaber, Paweł
dc.contributor.authorAdamus, Grażyna
dc.contributor.authorKowalczuk, Marek
dc.date.accessioned2018-07-04T14:39:15Z
dc.date.available2018-07-04T14:39:15Z
dc.date.issued2018-07-03
dc.description.abstractAn economically viable method to extract polyhydroxyalkanoates (PHAs) from cells is desirable for this biodegradable polymer of potential biomedical applications. In this work, two non-chlorinated solvents, cyclohexanone and γ-butyrolactone, were examined for extracting PHA produced by the bacterial strain Cupriavidus necator H16 cultivated on vegetable oil as a sole carbon source. The PHA produced was determined as a poly(3-hydroxybutyrate) (PHB) homopolyester. The extraction kinetics of the two solvents was determined using gel permeation chromatography (GPC). When cyclohexanone was used as the extraction solvent at 120 °C in 3 min, 95% of the PHB was recovered from the cells with a similar purity to that extracted using chloroform. With a decrease in temperature, the recovery yield decreased. At the same temperatures, the recovery yield of γ-butyrolactone was significantly lower. The effect of the two solvents on the quality of the extracted PHB was also examined using GPC and elemental analysis. The molar mass and dispersity of the obtained polymer were similar to that extracted using chloroform, while the nitrogen content of the PHB extracted using the two new solvents was slightly higher. In a nutshell, cyclohexanone in particular was identified as an expedient candidate to efficiently drive novel, sustainable PHA extraction processes.en_UK
dc.identifier.citationGuozhan Jiang, Brian Johnston, David E. Townrow, et al., Biomass extraction using non-chlorinated solvents for biocompatibility improvement of polyhydroxyalkanoates, Polymers, Volume 10, Issue 7, Article number 731en_UK
dc.identifier.issn2073-4360
dc.identifier.urihttps://doi.org/10.3390/polym10070731
dc.identifier.urihttp://dspace.lib.cranfield.ac.uk/handle/1826/13318
dc.language.isoenen_UK
dc.publisherMDPIen_UK
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectcyclohexanoneen_UK
dc.subjectγ-butyrolactoneen_UK
dc.subjectchloroformen_UK
dc.subjectextractionen_UK
dc.subjectpolyhydroxyalkanoatesen_UK
dc.subjectPHBen_UK
dc.titleBiomass extraction using non-chlorinated solvents for biocompatibility improvement of polyhydroxyalkanoatesen_UK
dc.typeArticleen_UK

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