Low sample volume origami-paper-based graphene-modified aptasensors for label-free electrochemical detection of cancer biomarker-EGFR

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dc.contributor.authorWang, Yang
dc.contributor.authorSun, Shuai
dc.contributor.authorLuo, Jinping
dc.contributor.authorXiong, Ying
dc.contributor.authorMing, Tao
dc.contributor.authorLiu, Juntao
dc.contributor.authorMa, Yuanyuan
dc.contributor.authorYan, Shi
dc.contributor.authorYang, Yue
dc.contributor.authorYang, Zhugen
dc.contributor.authorReboud, Julien
dc.contributor.authorYin, Huabing
dc.contributor.authorCooper, Jonathan M.
dc.contributor.authorCai, Xinxia
dc.date.accessioned2020-12-08T12:23:39Z
dc.date.available2020-12-08T12:23:39Z
dc.date.issued2020-05-18
dc.description.abstractIn this work, an electrochemical paper-based aptasensor was fabricated for label-free and ultrasensitive detection of epidermal growth factor receptor (EGFR) by employing anti-EGFR aptamers as the bio-recognition element. The device used the concept of paper-folding, or origami, to serve as a valve between sample introduction and detection, so reducing sampling volumes and improving operation convenience. Amino-functionalized graphene (NH2-GO)/thionine (THI)/gold particle (AuNP) nanocomposites were used to modify the working electrode not only to generate the electrochemical signals, but also to provide an environment conducive to aptamer immobilization. Electrochemical characterization revealed that the formation of an insulating aptamer–antigen immunocomplex would hinder electron transfer from the sample medium to the working electrode, thus resulting in a lower signal. The experimental results showed that the proposed aptasensor exhibited a linear range from 0.05 to 200 ngmL−1 (R2 = 0.989) and a detection limit of 5 pgmL−1 for EGFR. The analytical reliability of the proposed paper-based aptasensor was further investigated by analyzing serum samples, showing good agreement with the gold-standard enzyme-linked immunosorbent assayen_UK
dc.identifier.citationWang Y, Sun S, Luo J, et al., (2020) Low sample volume origami-paper-based graphene-modified aptasensors for label-free electrochemical detection of cancer biomarker-EGFR. Microsystems and Nanoengineering, Volume 6, Article number 32, 2020en_UK
dc.identifier.issn2055-7434
dc.identifier.urihttps://doi.org/10.1038/s41378-020-0146-2
dc.identifier.urihttp://dspace.lib.cranfield.ac.uk/handle/1826/16059
dc.language.isoenen_UK
dc.publisherNature Publishing Groupen_UK
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectNanoparticlesen_UK
dc.subjectMicrofluidicsen_UK
dc.subjectBiosensorsen_UK
dc.titleLow sample volume origami-paper-based graphene-modified aptasensors for label-free electrochemical detection of cancer biomarker-EGFRen_UK
dc.typeArticleen_UK

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