Phytotherapeutic analysis of chloroform-based fractions of Alstonia scholaris and Wrightia tinctoria extracts reveals potent anti-psoriatic activity: an in vitro and in vivo study

Simple item page
dc.contributor.authorOjha, Madhavi
dc.contributor.authorManocha, Nilanshu
dc.contributor.authorKumar, Vinod
dc.contributor.authorKarthikeyan, Ganeshan
dc.contributor.authorToor, Devinder
dc.date.accessioned2025-03-14T15:46:02Z
dc.date.available2025-03-14T15:46:02Z
dc.date.freetoread2025-03-14
dc.date.issued2025-03-01
dc.date.pubOnline2025-02-22
dc.description.abstractBackground/Objectives: Psoriasis, a prevalent dermatological disorder, poses therapeutic challenges due to limited effective treatments or adverse side-effects. Traditional medicinal plants like Alstonia scholaris and Wrightia tinctoria, historically used in Ayurvedic and Siddha practices, show potential in treating inflammatory skin diseases. This study aims to explore their in vitro and in vivo anti-psoriatic properties to develop safer and more effective therapies. Methods: Chloroform:methanol fractions from ethanol extracts of Alstonia scholaris and Wrightia tinctoria were evaluated for anti-psoriatic activity. In vitro assays using HaCaT cells assessed cell viability, apoptosis, and inflammatory markers. In vivo studies utilized an IMQ-induced psoriasis mouse model, evaluating skin lesions, histopathology, and cytokine profiles. Results: Chloroform fractions significantly reduced HaCaT cell viability and induced apoptosis. They also dose-dependently downregulated IL-8 and RANTES levels. In vivo, these fractions reduced skin inflammation, edema, and psoriasis scores. Histopathological analysis showed decreased epidermal thickness and dermal inflammation. Key psoriasis biomarkers IL-17 and IL-23 were significantly reduced. Conclusions: Chloroform:methanol fractions from Alstonia scholaris and Wrightia tinctoria demonstrated potent anti-psoriatic effects in vitro and in vivo. These findings support their potential as novel phytotherapeutic agents for managing psoriasis, offering promise for further development and clinical application.
dc.description.journalNamePharmaceuticals
dc.format.extentpp. 304-304
dc.identifier.citationOjha M, Manocha N, Kumar V, et al., (2025) Phytotherapeutic analysis of chloroform-based fractions of Alstonia scholaris and Wrightia tinctoria extracts reveals potent anti-psoriatic activity: an in vitro and in vivo study. Pharmaceuticals, Volume 18, Issue 3, March 2025, pp. 304-304
dc.identifier.eissn1424-8247
dc.identifier.elementsID565663
dc.identifier.issn1424-8247
dc.identifier.issueNo3
dc.identifier.urihttps://doi.org/10.3390/ph18030304
dc.identifier.urihttps://dspace.lib.cranfield.ac.uk/handle/1826/23613
dc.identifier.volumeNo18
dc.languageEnglish
dc.language.isoen
dc.publisherMDPI
dc.publisher.urihttps://www.mdpi.com/1424-8247/18/3
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject3214 Pharmacology and Pharmaceutical Sciences
dc.subject32 Biomedical and Clinical Sciences
dc.subjectComplementary and Integrative Health
dc.subjectAutoimmune Disease
dc.subjectPsoriasis
dc.subject5.1 Pharmaceuticals
dc.subjectSkin
dc.subjectInflammatory and immune system
dc.titlePhytotherapeutic analysis of chloroform-based fractions of Alstonia scholaris and Wrightia tinctoria extracts reveals potent anti-psoriatic activity: an in vitro and in vivo study
dc.typeArticle
dcterms.dateAccepted2025-02-18

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Phytotherapeutic_analysis_of_chloroform-based_fractions-2025.pdf
Size:
15.29 MB
Format:
Adobe Portable Document Format
Description:
Published version
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.63 KB
Format:
Plain Text
Description:
Download

Collections

Staff publications (SWEE)