In Vivo water dynamics in Shewanella oneidensis bacteria at high pressure

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dc.contributor.authorFoglia, Fabrizia
dc.contributor.authorHazael, Rachael
dc.contributor.authorMeersman, Filip
dc.contributor.authorWilding, Martin C.
dc.contributor.authorSakai, Victoria García
dc.contributor.authorRogers, Sarah
dc.contributor.authorBove, Livia E.
dc.contributor.authorKoza, Michael Marek
dc.contributor.authorMoulin, Martine
dc.contributor.authorHaertlein, Michael
dc.contributor.authorForsyth, V. Trevor
dc.contributor.authorMcMillan, Paul F.
dc.date.accessioned2019-07-02T14:55:53Z
dc.date.available2019-07-02T14:55:53Z
dc.date.issued2019-06-18
dc.description.abstractFollowing observations of survival of microbes and other life forms in deep subsurface environments it is necessary to understand their biological functioning under high pressure conditions. Key aspects of biochemical reactions and transport processes within cells are determined by the intracellular water dynamics. We studied water diffusion and rotational relaxation in live Shewanella oneidensis bacteria at pressures up to 500 MPa using quasi-elastic neutron scattering (QENS). The intracellular diffusion exhibits a significantly greater slowdown (by −10–30%) and an increase in rotational relaxation times (+10–40%) compared with water dynamics in the aqueous solutions used to resuspend the bacterial samples. Those results indicate both a pressure-induced viscosity increase and slowdown in ionic/macromolecular transport properties within the cells affecting the rates of metabolic and other biological processes. Our new data support emerging models for intracellular organisation with nanoscale water channels threading between macromolecular regions within a dynamically organized structure rather than a homogenous gel-like cytoplasm.en_UK
dc.identifier.citationFoglia F, Hazael R, Meersman F, Wilding MC, García Sakai V, Rogers S, Bove LE, Marek Koza M, Moulin M, Haertlein M, Forsyth VT and McMillan PF., In Vivo water dynamics in Shewanella oneidensis bacteria at high pressure, Scientific Reports, Volume 9, Article Number 8716en_UK
dc.identifier.issn2045-2322
dc.identifier.urihttps://doi.org/10.1038/s41598-019-44704-3
dc.identifier.urihttp://dspace.lib.cranfield.ac.uk/handle/1826/14283
dc.language.isoenen_UK
dc.publisherNature Publishing Groupen_UK
dc.relation.ispartofseries;8716
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleIn Vivo water dynamics in Shewanella oneidensis bacteria at high pressureen_UK
dc.typeArticleen_UK

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