Mycotoxin production of Alternaria strains isolated from Korean barley grains determined by LC-MS/MS

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dc.contributor.authorNguyen, Thuong T. T.
dc.contributor.authorKim, Jueun
dc.contributor.authorJeon, Sun Jeong
dc.contributor.authorLee, Chul Won
dc.contributor.authorMagan, Naresh
dc.contributor.authorLee, Hyang Burm
dc.date.accessioned2018-02-21T11:29:24Z
dc.date.available2018-02-21T11:29:24Z
dc.date.issued2018-01-03
dc.description.abstractTwenty-four Alternaria strains were isolated from barley grain samples. These strains were screened for the production of mycotoxins on rice medium using thin layer chromatography. All 24 strains produced at least one of the five mycotoxins (ALT, AOH, ATX-I, AME, and TeA). Three representative strains, namely EML-BLDF1-4, EML-BLDF1-14, and EML-BLDF1-18, were further analyzed using a new LC–MS/MS-based mycotoxin quantification method. This method was used to detect and quantify Alternaria mycotoxins. We used positive ion electrospray mass spectrometry with multiple reaction mode (MRM) for the simultaneous quantification of various Alternaria mycotoxins produced by these strains. Five Alternaria toxins (ALT, ATX-I, AOH, AME, and TeA) were detected and quantified. Sample preparation included methanol extraction, concentration, and injection into LC–MS/MS. Limit of detection ranged from 0.13 to 4 μg/mL and limit of quantification ranged from 0.25 to 8 μg/mL.en_UK
dc.identifier.citationNguyen TT, Kim J, Jeon SJ, Lee CW, Magan N, Lee HB, Mycotoxin production of Alternaria strains isolated from Korean barley grains determined by LC-MS/MS, International Journal of Food Microbiology, Volume 268, 2 March 2018, pp. 44-52en_UK
dc.identifier.issn0168-1605
dc.identifier.urihttp://dx.doi.org/10.1016/j.ijfoodmicro.2018.01.003
dc.identifier.urihttp://dspace.lib.cranfield.ac.uk/handle/1826/13021
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectAlternaria mycotoxinsen_UK
dc.subjectAlternaria strainsen_UK
dc.subjectSimultaneous quantificationen_UK
dc.subjectDeterminationen_UK
dc.subjectAnalysis conditionsen_UK
dc.subjectLC-MS/MSen_UK
dc.titleMycotoxin production of Alternaria strains isolated from Korean barley grains determined by LC-MS/MSen_UK
dc.typeArticleen_UK

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