CRISPR-enabled genetic logic circuits for biosensing

Date published

2025-09-01

Free to read from

2025-05-29

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Journal Title

Journal ISSN

Volume Title

Publisher

Elsevier

Department

Type

Article

ISSN

0165-9936

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Citation

Wang X, Gao Y, Zhou N, et al., (2025) CRISPR-enabled genetic logic circuits for biosensing. TrAC Trends in Analytical Chemistry, Volume 190, May 2025, Article number 118297

Abstract

Synthetic biology aims to engineer genetic circuits for custom-designed behaviors in living systems, including sophisticated biosensing applications. The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system has gained attention for its potential in genetic circuit design due to its modularity, programmability, precision, and orthogonality. Here we highlight the current CRISPR-based tools for gene regulation at both transcriptional and translational levels. We discuss how these CRISPR technologies facilitate the design and construction of complex genetic circuits that can perform customized logic computations within living systems. Furthermore, we summarize the applications of CRISPR-based genetic logic circuits in biosensing, emphasizing their potential for detecting diverse biological and environmental signals. Finally, we highlight the key challenges facing the development and application of CRISPR-enabled genetic logic circuits and propose directions for future research to overcome these bottlenecks.

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Software Description

Software Language

Github

Keywords

3401 Analytical Chemistry, 34 Chemical Sciences, Biotechnology, Bioengineering, Genetics

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Attribution 4.0 International

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Funder/s

This work was supported by National Natural Science Foundation of China (32320103001, 32271475), “Pioneer” and “Leading Goose” R&D Program of Zhejiang (2024C03011), National Key R&D Program of China (2023YFF1204500), Beijing Life Science Academy (2024200CA0070), Kunpeng Action Program Award of Zhejiang Province, and China Postdoctoral Science Foundation (2022M722780). ZY thanks Leverhulme Trust Research Leadership Award (RL-2022-041) and Leverhulme Trust Visiting Professorship grant (VP1-2024-030).