Fermentative production of β-Carotene from sugarcane bagasse hydrolysate by Rhodotorula glutinis CCT-2186

dc.contributor.authorDíaz-Ruiz, Erick
dc.contributor.authorBalbino, Thércia R.
dc.contributor.authordos Santos, Júlio C.
dc.contributor.authorKumar, Vinod
dc.contributor.authorda Silva, Silvio S.
dc.contributor.authorChandel, Anuj K.
dc.date.accessioned2023-11-22T15:31:45Z
dc.date.available2023-11-22T15:31:45Z
dc.date.issued2023-11-02
dc.description.abstractΒ-Carotene is a red–orange pigment that serves as a precursor to important pharmaceutical molecules like vitamin A and retinol, making it highly significant in the industrial sector. Consequently, there is an ongoing quest for more sustainable production methods. In this study, glucose and xylose, two primary sugars derived from sugarcane bagasse (SCB), were utilized as substrates for β-carotene production by Rhodotorula glutinis CCT-2186. To achieve this, SCB underwent pretreatment using NaOH, involved different concentrations of total solids (TS) (10%, 15%, and 20%) to remove lignin. Each sample was enzymatically hydrolyzed using two substrate loadings (5% and 10%). The pretreated SCB with 10%, 15%, and 20% TS exhibited glucose hydrolysis yields (%wt) of 93.10%, 91.88%, and 90.77%, respectively. The resulting hydrolysate was employed for β-carotene production under batch fermentation. After 72 h of fermentation, the SCB hydrolysate yielded a β-carotene concentration of 118.56 ± 3.01 mg/L. These findings showcase the robustness of R. glutinis as a biocatalyst for converting SCB into β-carotene.en_UK
dc.identifier.citationDíaz-Ruiz E, Balbino TR, Dos Santos JC, et al., (2023) Fermentative production of β-Carotene from sugarcane bagasse hydrolysate by Rhodotorula glutinis CCT-2186, Applied Biochemistry and Biotechnology, Available online 02 November 2023en_UK
dc.identifier.eissn1559-0291
dc.identifier.issn0273-2289
dc.identifier.urihttps://doi.org/10.1007/s12010-023-04761-x
dc.identifier.urihttps://dspace.lib.cranfield.ac.uk/handle/1826/20575
dc.language.isoenen_UK
dc.publisherSpringeren_UK
dc.subjectSugarcane bagasseen_UK
dc.subjectPretreatmenten_UK
dc.subjectEnzymatic hydrolysisen_UK
dc.subjectRhodotorula glutinisen_UK
dc.subjectFermentationen_UK
dc.subjectβ-Caroteneen_UK
dc.titleFermentative production of β-Carotene from sugarcane bagasse hydrolysate by Rhodotorula glutinis CCT-2186en_UK
dc.typeArticleen_UK

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