FABP4-mediated lipid droplet formation in Streptococcus uberis-infected macrophages supports host defence

Simple item page
dc.contributor.authorWan, Zhixin
dc.contributor.authorFu, Shaodong
dc.contributor.authorWang, Zhenglei
dc.contributor.authorXu, Yuanyuan
dc.contributor.authorZhou, Yuanyuan
dc.contributor.authorLin, Xinguang
dc.contributor.authorLan, Riguo
dc.contributor.authorHan, Xiangan
dc.contributor.authorLuo, Zhenhua
dc.contributor.authorMiao, Jinfeng
dc.date.accessioned2022-11-23T16:56:11Z
dc.date.available2022-11-23T16:56:11Z
dc.date.issued2022-11-12
dc.description.abstractFoamy macrophages containing prominent cytoplasmic lipid droplets (LDs) are found in a variety of infectious diseases. However, their role in Streptococcus uberis-induced mastitis is unknown. Herein, we report that S. uberis infection enhances the fatty acid synthesis pathway in macrophages, resulting in a sharp increase in LD levels, accompanied by a significantly enhanced inflammatory response. This process is mediated by the involvement of fatty acid binding protein 4 (FABP4), a subtype of the fatty acid-binding protein family that plays critical roles in metabolism and inflammation. In addition, FABP4 siRNA inhibitor cell models showed that the deposition of LDs decreased, and the mRNA expression of Tnf, Il1b and Il6 was significantly downregulated after gene silencing. As a result, the bacterial load in macrophages increased. Taken together, these data demonstrate that macrophage LD formation is a host-driven component of the immune response to S. uberis. FABP4 contributes to promoting inflammation via LDs, which should be considered a new target for drug development to treat infections.en_UK
dc.identifier.citationWan Z, Fu S, Wang Z, et al., (2022) FABP4-mediated lipid droplet formation in Streptococcus uberis-infected macrophages supports host defence. Veterinary Research, Volume 53, November 2022, Article number 90en_UK
dc.identifier.issn0928-4249
dc.identifier.urihttps://doi.org/10.1186/s13567-022-01114-0
dc.identifier.urihttps://dspace.lib.cranfield.ac.uk/handle/1826/18737
dc.language.isoenen_UK
dc.publisherBMC - Springeren_UK
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectFABP4en_UK
dc.subjectlipid dropletsen_UK
dc.subjectStreptococcus uberisen_UK
dc.subjectbovine mastitisen_UK
dc.subjectinflammationen_UK
dc.titleFABP4-mediated lipid droplet formation in Streptococcus uberis-infected macrophages supports host defenceen_UK
dc.typeArticleen_UK

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
FABP4-mediated_lipid_droplet_formation-2022.pdf
Size:
6.81 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.63 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Staff publications (SWEE)