Highly sensitive optical fibre long period grating biosensor anchored with silica core gold shell nanoparticles

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dc.contributor.authorMarques, L.
dc.contributor.authorHernandez, F. U.
dc.contributor.authorJames, Stephen W.
dc.contributor.authorMorgan, S. P.
dc.contributor.authorClark, M.
dc.contributor.authorTatam, Ralph P.
dc.contributor.authorKorposh, Sergiy
dc.date.accessioned2016-08-25T15:40:52Z
dc.date.available2016-08-25T15:40:52Z
dc.date.issued2015-08-22
dc.description.abstractAn optical fibre long period grating (LPG), modified with a coating of silica core gold shell (SiO2:Au) nanoparticles (NPs) deposited using the layer-by-layer method, was employed for the development of a biosensor. The SiO2:Au NPs were electrostatically assembled onto the LPG with the aid of a poly(allylamine hydrochloride) (PAH) polycation layer. The LPG sensor operates at the phase matching turning point to provide the highest sensitivity. The SiO2:Au NPs were modified with biotin, which was used as a ligand for streptavidin (SV) detection. The sensing mechanism is based on the measurement of the refractive index change induced by the binding of the SV to the biotin. The effect on sensitivity of increasing the surface area by virtue of the SiO2:Au nanoparticles’ diameter and film thickness was studied. The lowest measured concentration of SV was 2.5 nM, achieved using an LPG modified with a 3 layer (PAH/SiO2:Au) thin film composed of SiO2 NPs of 300 nm diameter with a binding constant of View the MathML sourcek=1.7(pM)-1, sensitivity of View the MathML source6.9nm/ng/mm2 and limit of detection of 19 pg/mm2.en_UK
dc.identifier.citationMarques L, Hernandez FU, James SW, et al., (2016) Highly sensitive optical fibre long period grating biosensor anchored with silica core gold shell nanoparticles. Biosensors and Bioelectronics, Volume 75, January 2016, pp. 222-231en_UK
dc.identifier.cris5775379
dc.identifier.issn0956-5663
dc.identifier.urihttps://doi.org/10.1016/j.bios.2015.08.046
dc.identifier.urihttps://dspace.lib.cranfield.ac.uk/handle/1826/10454
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectLong period gratingen_UK
dc.subjectBiosensoren_UK
dc.subjectAntigen–antibodyen_UK
dc.subjectSilica-gold core/shell nanoparticlesen_UK
dc.subjectLayer-by-layeren_UK
dc.titleHighly sensitive optical fibre long period grating biosensor anchored with silica core gold shell nanoparticlesen_UK
dc.typeArticleen_UK

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