A loss-of-function allele of a TAC1-like gene (SlTAC1) located on tomato chromosome 10 is a candidate for the Erectoid leaf (Erl) mutation

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dc.contributor.authorGonzález-Arcos, Matías
dc.contributor.authorde Noronha Fonseca, Maria Esther
dc.contributor.authorBasílio Zandonadi, Daniel
dc.contributor.authorPeres, Lázaro E. P.
dc.contributor.authorArruabarrena, Ana
dc.contributor.authorFerreira, Demetryus S.
dc.contributor.authorKevei, Zoltan
dc.contributor.authorMohareb, Fady R.
dc.contributor.authorThompson, Andrew J.
dc.contributor.authorBoiteux, Leonardo S.
dc.date.accessioned2019-04-30T11:31:18Z
dc.date.available2019-04-30T11:31:18Z
dc.date.issued2019-04-16
dc.description.abstractThe genetic basis of an erectoid leaf phenotype was investigated in distinct tomato breeding populations, including one derived from Solanum lycopersicum ‘LT05’ (with the erectoid leaf phenotype and uniform ripening, genotype uu) × S. pimpinellifollium ‘TO-937’ (with the wild-type leaf phenotype and green fruit shoulder, genotype UU). The erectoid leaf phenotype was inherited as a semi-dominant trait and it co-segregated with the u allele of gene SlGLK2 (Solyc10g008160). This genomic location coincides with a previously described semi-dominant mutation named as Erectoid leaf (Erl). The genomes of ‘LT05’, ‘TO-937’, and three other unrelated accessions (with the wild-type Erl+ allele) were resequenced with the aim of identifying candidate genes. Comparative genomic analyses, including the reference genome ‘Heinz 1706’ (Erl+ allele), identified an Erectoid leaf-specific single nucleotide polymorphism (SNP) in the gene Solyc10g009320. This SNP caused a change of a glutamine codon (present in all the wild-type genomes) to a TAA (= ochre stop-codon) in the Erl allele, resulting in a smaller version of the predicted mutant protein (221 vs. 279 amino acids). Solyc10g009320, previously annotated as an ‘unknown protein’, was identified as a TILLER ANGLE CONTROL1-like gene. Linkage between the Erl and Solyc10g009320 was confirmed via Sanger sequencing of the PCR amplicons of the two variant alleles. No recombinants were detected in 265 F2 individuals. Contrasting S7 near-isogenic lines were also homozygous for each of the alternate alleles, reinforcing Solyc10g009320 as a strong Erl candidate gene and opening the possibility for fine-tuning manipulation of tomato architecture in breeding programs.en_UK
dc.identifier.citationGonzález-Arcos M, de Noronha Fonseca ME, Zandonadi DB, Peres LE, Arruabarrena A, Ferreira DS, Kevei Z, Mohareb F, Thompson AJ and Boiteux LS, A loss-of-function allele of a TAC1-like gene (SlTAC1) located on tomato chromosome 10 is a candidate for the Erectoid leaf (Erl) mutation. Euphytica, Volume 215, May 2019, Article number 95en_UK
dc.identifier.issn0014-2336
dc.identifier.urihttps://doi.org/10.1007/s10681-019-2418-1
dc.identifier.urihttp://dspace.lib.cranfield.ac.uk/handle/1826/14125
dc.language.isoenen_UK
dc.publisherSpringer Verlagen_UK
dc.rightsAttribution-NonCommercial 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/*
dc.subjectSolanum lycopersicumen_UK
dc.subjectResequencingen_UK
dc.subjectComparative genomic analysisen_UK
dc.subjectPlant architectureen_UK
dc.subjectBreedingen_UK
dc.titleA loss-of-function allele of a TAC1-like gene (SlTAC1) located on tomato chromosome 10 is a candidate for the Erectoid leaf (Erl) mutationen_UK
dc.typeArticleen_UK

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