Direct replacement of antibodies with molecularly imprinted polymer (MIP) nanoparticles in ELISA - development of a novel assay for vancomycin
| dc.contributor.author | Chianella, Iva | - |
| dc.contributor.author | Guerreiro, Antonio R. | - |
| dc.contributor.author | Moczko, Ewa | - |
| dc.contributor.author | Caygill, J. S. | - |
| dc.contributor.author | Piletska, Elena V. | - |
| dc.contributor.author | Perez De Vargas Sansalvador, Isabel M. | - |
| dc.contributor.author | Whitcombe, Michael J. | - |
| dc.contributor.author | Piletsky, Sergey A. | - |
| dc.date.accessioned | 2014-09-02T04:01:23Z | |
| dc.date.available | 2014-09-02T04:01:23Z | |
| dc.date.issued | 2013-09-03T00:00:00Z | - |
| dc.description.abstract | A simple and straightforward technique for coating microplate wells with molecularly imprinted polymer nanoparticles (nanoMIPs) to develop ELISA type assays is presented here for the first time. NanoMIPs were synthesized by a solid phase approach with immobilized vancomycin (template) and characterized using Biacore 3000, dynamic light scattering and electron microscopy. Immobilization, blocking and washing conditions were optimized in microplate format. The detection of vancomycin was achieved in competitive binding experiments with a HRP-vancomycin conjugate. The assay was capable of measuring vancomycin in buffer and in blood plasma within the range 0.001-70 nM with a detection limit of 0.0025 nM (2.5 pM). The sensitivity of the assay was three orders of magnitude better than a previously described ELISA based on antibodies. In these experiments nanoMIPs have shown high affinity and minimal interference from blood plasma components. Immobilized nanoMIPs were stored for 1 month at room temperature without any detrimental effects to their binding properties. The high affinity of nanoMIPs and the lack of a requirement for cold chain logistics make them an attractive alternative to traditional antibodies used in ELISA | en_UK |
| dc.identifier.citation | Iva Chianella, Antonio Guerreiro, Ewa Moczko, J. Sarah Caygill, Elena V. Piletska, Isabel M. Perez De Vargas Sansalvador, Michael J. Whitcombe and Sergey A. Piletsky, Direct replacement of antibodies with molecularly imprinted polymer (MIP) nanoparticles in ELISA - development of a novel assay for vancomycin, Analytical Chemistry, 2013, Volume 85, Issue 17, Pages 8462–8468. | |
| dc.identifier.issn | 0003-2700 | - |
| dc.identifier.uri | http://dx.doi.org/10.1021/ac402102j | - |
| dc.identifier.uri | http://dspace.lib.cranfield.ac.uk/handle/1826/8663 | |
| dc.language.iso | en_UK | - |
| dc.publisher | ACS American Chemical Society | en_UK |
| dc.rights | Journal acknowledges that Author retains the right to provide a copy of the final peer-reviewed manuscript to the NIH upon acceptance for Journal publication, for public archiving in PubMed Central as soon as possible but no later than 12 months after publication by Journal | |
| dc.title | Direct replacement of antibodies with molecularly imprinted polymer (MIP) nanoparticles in ELISA - development of a novel assay for vancomycin | en_UK |
| dc.type | Article | - |