DBT degradation enhancement by decorating Rhodococcus erythropolis IGST8 with magnetic Fe3O4 nanoparticles

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dc.contributor.authorAnsari, Farahnaz
dc.contributor.authorGrigoriev, P.
dc.contributor.authorLibor, Zsuzsanna
dc.contributor.authorTothill, Ibtisam E.
dc.contributor.authorRamsden, Jeremy J.
dc.date.accessioned2010-05-06T09:13:20Z
dc.date.available2010-05-06T09:13:20Z
dc.date.issued2009-04
dc.description.abstractBiodesulfurization (BDS) of dibenzothiophene (DBT) was carried out by Rhodococcus erythropolis IGST8 decorated with magnetic Fe3O4 nanoparticles, synthesized in-house by a chemical method, with an average size of 45-50 nm, in order to facilitate the post-reaction separation of the bacteria from the reaction mixture. Scanning electron microscopy (SEM) showed that the magnetic nanoparticles substantially coated the surfaces of the bacteria. It was found that the decorated cells had a 56% higher DBT desulfurization activity in basic salt medium (BSM) compared to the nondecorated cells. We propose that this is due to permeabilization of the bacterial membrane, facilitating the entry and exit of reactant and product, respectively. Model experiments with black lipid membranes (BLM) demonstrated that the nanoparticles indeed enhance membrane permeability.en_UK
dc.identifier.citationAnsari F, Grigoriev P, Libor S, et al., (2009) DBT degradation enhancement by decorating Rhodococcus erythropolis IGST8 with magnetic Fe3O4 nanoparticles. Biotechnology and Bioengineering, Volume 102, Issue 5,April 2009, pp. 1505-1512en_UK
dc.identifier.issn0006-3592
dc.identifier.urihttp://dx.doi.org/10.1002/bit.22161
dc.identifier.urihttp://hdl.handle.net/1826/4400
dc.language.isoenen_UK
dc.publisherWiley-Blackwellen_UK
dc.rightsThe definitive version is available at www3.interscience.wiley.com
dc.subjectBiodesulfurizationen_UK
dc.subjectDibenzothiopheneen_UK
dc.subjectRhodococcus erythropolisen_UK
dc.subjectMagnetic Fe3O4en_UK
dc.subjectNanoparticlesen_UK
dc.subjectBlack lipid membraneen_UK
dc.titleDBT degradation enhancement by decorating Rhodococcus erythropolis IGST8 with magnetic Fe3O4 nanoparticlesen_UK
dc.typeArticleen_UK

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