Efficient DNA walker guided by ordered cruciform-shaped DNA track for ultrasensitive and rapid electrochemical detection of lead ion

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dc.contributor.authorZhu, Nuanfei
dc.contributor.authorWang, Kaixuan
dc.contributor.authorXiong, Dinghui
dc.contributor.authorXiao, Jiaxuan
dc.contributor.authorDeng, Yibin
dc.contributor.authorYang, Zhugen
dc.contributor.authorZhang, Zhen
dc.date.accessioned2024-04-19T10:59:52Z
dc.date.available2024-04-19T10:59:52Z
dc.date.issued2024-03-20
dc.description.abstractThe rational design of DNA tracks is an effective pathway to guide the autonomous movement and high-efficiency recognition in DNA walkers, showing outstanding advantages for the cascade signal amplification of electrochemical biosensors. However, the uncontrolled distance between two adjacent tracks on the electrode could increase the risk of derailment and interruption of the reaction. Hence, a novel four-way balanced cruciform-shaped DNA track (C-DNT) was designed as a structured pathway to improve the effectiveness and stability of the reaction in DNA walkers. In this work, two kinds of cruciform-shaped DNA were interconnected as a robust structure that could avoid the invalid movement of the designed DNA walker on the electrode. When hairpin H2 was introduced onto the electrode, the strand displacement reaction (SDR) effectively triggered movements of the DNA walker along the cruciform-shaped track while leaving ferrocene (Fc) on the electrode, leading to a significant enhancement of the electrochemical signal. This design enabled the walker to move in an excellent organized and controllable manner, thus enhancing the reaction speed and walking efficiency. Compared to other walkers moving on random tracks, the reaction time of the C-DNT-based DNA walker could be reduced to 20 min. Lead ion (Pb2+) was used as a model target to evaluate the analytical performance of this biosensor, which exhibited a low detection limit of 0.033 pM along with a wide detection ranging from 0.1 pM to 500 nM. This strategy presented a novel concept for designing a high-performance DNA walker-based sensing platform for the detection of contaminants.en_UK
dc.identifier.citationZhu N, Wang K, Xiong D, et al., (2024) Efficient DNA walker guided by ordered cruciform-shaped DNA track for ultrasensitive and rapid electrochemical detection of lead ion. Analytica Chimica Acta, Volume 1302, May 2024, Article number 342492en_UK
dc.identifier.issn0003-2670
dc.identifier.urihttps://doi.org/10.1016/j.aca.2024.342492
dc.identifier.urihttps://dspace.lib.cranfield.ac.uk/handle/1826/21229
dc.language.isoen_UKen_UK
dc.publisherElsevieren_UK
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectBiosensoren_UK
dc.subjectElectrochemical analysisen_UK
dc.subjectDNA walkeren_UK
dc.subjectHeavy metalen_UK
dc.subjectRapid detectionen_UK
dc.titleEfficient DNA walker guided by ordered cruciform-shaped DNA track for ultrasensitive and rapid electrochemical detection of lead ionen_UK
dc.typeArticleen_UK
dcterms.dateAccepted2024-03-16

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