Pore-scale monitoring of the effect of microarchitecture on fungal growth in a two-dimensional soil-like micromodel

Simple item page
dc.contributor.authorSoufan, Raghad
dc.contributor.authorDelaunay, Yolaine
dc.contributor.authorVieublé Gonod, Laure
dc.contributor.authorShor, Leslie M.
dc.contributor.authorGarnier, Patricia
dc.contributor.authorOtten, Wilfred
dc.contributor.authorBaveye, Philippe C.
dc.date.accessioned2018-08-31T09:13:06Z
dc.date.available2018-08-31T09:13:06Z
dc.date.issued2018-07-03
dc.description.abstractIn spite of the very significant role that fungi are called to play in agricultural production and climate change over the next two decades, very little is known at this point about the parameters that control the spread of fungal hyphae in the pore space of soils. Monitoring of this process in 3 dimensions is not technically feasible at the moment. The use of transparent micromodels simulating the internal geometry of real soils affords an opportunity to approach the problem in 2 dimensions, provided it is confirmed that fungi would actually want to propagate in such artificial systems. In this context, the key objectives of the research described in this article are to ascertain, first, that the fungus Rhizoctonia solani can indeed grow in a micromodel of a sandy loam soil, and, second, to identify and analyze in detail the pattern by which it spreads in the tortuous pores of the micromodel. Experimental observations show that hyphae penetrate easily inside the micromodel, where they bend frequently to adapt to the confinement to which they are subjected, and branch at irregular intervals, unlike in current computer models of the growth of hyphae, which tend to describe them as series of straight tubular segments. A portion of the time, hyphae in the micromodels also exhibit thigmotropism, i.e., tend to follow solid surfaces closely. Sub-apical branching, which in unconfined situations seems to be controlled by the fungus, appears to be closely connected with the bending of the hyphae, resulting from their interactions with surfaces. These different observations not only indicate different directions to follow to modify current mesoscopic models of fungal growth, so they can apply to soils, but they also suggest a wealth of further experiments using the same set-up, involving for example competing fungal hyphae, or the coexistence of fungi and bacteria in the same pore space.en_UK
dc.identifier.citationRaghad Soufan, Yolaine Delaunay, Laure Vieublé Gonod, et al., Pore-scale monitoring of the effect of microarchitecture on fungal growth in a two-dimensional soil-like micromodel. Frontiers in Environmental Science, Online 3 July 2018, article number 68en_UK
dc.identifier.issn2296-665X
dc.identifier.urihttps://doi.org/10.3389/fenvs.2018.00068
dc.identifier.urihttp://dspace.lib.cranfield.ac.uk/handle/1826/13442
dc.language.isoenen_UK
dc.publisherFrontiers Mediaen_UK
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjecthyphaeen_UK
dc.subjectspreaden_UK
dc.subjectmicrofluidicsen_UK
dc.subjectfungal highwayen_UK
dc.subjectmicroscaleen_UK
dc.titlePore-scale monitoring of the effect of microarchitecture on fungal growth in a two-dimensional soil-like micromodelen_UK
dc.typeArticleen_UK

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
effect_of_microarchitecture_on_fungal_growth-2018.pdf
Size:
1.19 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.63 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Staff publications (SWEE)