Flexible Ultrathin PolyDVB/EVB Composite Membranes for the Optimization of a Lactate Sensor.

An ultrathin composite membrane has been developed as the outer covering barrier in a model amperometric lactate oxidase enzyme electrode. The membrane was formed by cathodic electropolymerization of divinylbenzene/ethylvinylbenzene at the surface of a gold coated polyester support membrane. Permeability coefficients were determined for O2 and lactate across membranes with a range of polymer thicknesses. Anionic interferents (such as ascorbate) were screened from the working electrode by the composite membrane. The composite enzyme electrode showed an increased working concentration range and extended linearity of responses in comparison to an uncoated enzyme electrode.