Effects of osmotic and matric potential on radial growth and accumulation of endogenous reserves in three isolates of Pochonia chlamydosporia

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dc.contributor.authorEsteves, Ivania
dc.contributor.authorPeteira, Belkis
dc.contributor.authorPowers, S.
dc.contributor.authorMagan, Naresh
dc.contributor.authorKerry, Brian R.
dc.date.accessioned2010-04-29T14:10:11Z
dc.date.available2010-04-29T14:10:11Z
dc.date.issued2009-02
dc.description.abstractFor the first time, the effects of varying osmotic and matric potential on fungal radial growth and accumulation of polyols were studied in three isolates of Pochonia chlamydosporia. Fungal radial growth was measured on potato dextrose agar modified osmotically using potassium chloride or glycerol. PEG 8000 was used to modify matric potential. When plotted, the radii of the colonies were found to grow linearly with time, and regression was applied to estimate the radial growth rate (mm day-1). Samples of fresh mycelia from 25-day-old cultures were collected and the quantity (mg g-1 fresh biomass) of four polyols (glycerol, erythritol, arabitol and mannitol) and one sugar (glucose) was determined using HPLC. Results revealed that fungal radial growth rates decreased with increased osmotic or matric stress. Statistically significant differences in radial growth were found between isolates in response to matric stress (P<0.006) but not in response to osmotic stress (P=0.759). Similarly, differences in the total amounts of polyols accumulated by the fungus were found between isolates in response to matric stress (P<0.001), but not in response to osmotic stress (P=0.952). Under water stress, the fungus accumulated a combination of different polyols important in osmoregulation, which depended on the solute used to generate the stress. Arabitol and glycerol were the main polyols accumulated in osmotically modified media, whereas erythritol was the main polyol that was accumulated in media amended with PEG. The results found that Pochonia chlamydosporia may use different osmoregulation mechanisms to overcome osmotic and matric stresses.en_UK
dc.identifier.citationI. Esteves; B. Peteira; S. Powers; N. Magan; B. R. Kerry; Effects of osmotic and matric potential on radial growth and accumulation of endogenous reserves in three isolates of Pochonia chlamydosporia, Biocontrol Science and Technology, Volume 19, Issue 2, February 2009, pages 185-199en_UK
dc.identifier.issn0958-3157
dc.identifier.urihttp://dx.doi.org/10.1080/09583150802650175
dc.identifier.urihttp://hdl.handle.net/1826/4392
dc.language.isoenen_UK
dc.publisherTaylor and Francisen_UK
dc.subjectbiological controlen_UK
dc.subjectwater stressen_UK
dc.subjectPochonia chlamydosporiaen_UK
dc.subjectroot-knot nematodesen_UK
dc.subjectcyst nematodesen_UK
dc.subjectendogenous reservesen_UK
dc.titleEffects of osmotic and matric potential on radial growth and accumulation of endogenous reserves in three isolates of Pochonia chlamydosporiaen_UK
dc.typeArticleen_UK

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