Detection of β-amyloid peptide (1–16) and amyloid precursor protein (APP770) using spectroscopic ellipsometry and QCM techniques: A step forward towards Alzheimers disease diagnostics
| dc.contributor.author | Mustafa, M. K. | |
| dc.contributor.author | Nabok, Alexei | |
| dc.contributor.author | Parkinson, D. | |
| dc.contributor.author | Tothill, Ibtisam E. | |
| dc.contributor.author | Salam, Faridah | |
| dc.contributor.author | Tsargorodskaya, A. | |
| dc.date.accessioned | 2011-01-24T15:03:57Z | |
| dc.date.available | 2011-01-24T15:03:57Z | |
| dc.date.issued | 2010-12-15 | |
| dc.description.abstract | A highly sensitive method of spectroscopic ellipsometry in total internal reflection mode (TIRE) was exploited for detecting β-amyloid peptide (Aβ1–16) in the direct immune reaction with monoclonal DE2 antibodies (raised against Aβ1–16) electrostatically immobilised on the surface of gold. A rapid detection of Aβ1–16 in a wide range of concentrations from 5 μg/ml down to 0.05 ng/ml was achieved using a cost-effective and label-free direct immunoassay format. TIRE dynamic spectral measurements proved that the immune reaction between DE2 monoclonal antibodies and Aβ1–16 is highly specific with the affinity constant KD = 1.46 × 10−8 mol/l. The same DE2 antibodies were utilised for detection of amyloid precursor protein APP770, a larger protein containing Aβ1–16 domain, using the quartz crystal microbalance (QCM) measurements in liquid. A combination of QCM and TIRE kinetics results allowed the evaluation of the originally unknown concentration of APP770 in complete medium solution containing other proteins, salts, and amino acids. | en_UK |
| dc.identifier.citation | M.K. Mustafa, A. Nabok, D. Parkinson, I.E. Tothill, F. Salam and A. Tsargorodskaya, Detection of β-amyloid peptide (1–16) and amyloid precursor protein (APP770) using spectroscopic ellipsometry and QCM techniques: A step forward towards Alzheimers disease diagnostics, Biosensors and Bioelectronics, Volume 26, Issue 4, 15 December 2010, Pages 1332-1336 | en_UK |
| dc.identifier.issn | 0956-5663 | |
| dc.identifier.uri | http://dx.doi.org/10.1016/j.bios.2010.07.042 | |
| dc.identifier.uri | http://dspace.lib.cranfield.ac.uk/handle/1826/4760 | |
| dc.language.iso | en | en_UK |
| dc.publisher | Elsevier | en_UK |
| dc.subject | Alzheimer's disease | en_UK |
| dc.subject | β-Amyloid peptide 1–16 | en_UK |
| dc.subject | Amyloid precursor protein (APP770) | en_UK |
| dc.subject | Direct immunoassay | en_UK |
| dc.subject | Total internal reflection ellipsometry | en_UK |
| dc.subject | Quartz crystal microbalance | en_UK |
| dc.title | Detection of β-amyloid peptide (1–16) and amyloid precursor protein (APP770) using spectroscopic ellipsometry and QCM techniques: A step forward towards Alzheimers disease diagnostics | en_UK |
| dc.type | Article | en_UK |