Development of an immunosensor for PfHRP 2 as a biomarker for Malaria detection
| dc.contributor.author | Hemben, Aver | |
| dc.contributor.author | Ashley, Jon | |
| dc.contributor.author | Tothill, Ibtisam E. | |
| dc.date.accessioned | 2017-07-19T16:07:36Z | |
| dc.date.available | 2017-07-19T16:07:36Z | |
| dc.date.issued | 2017-07-18 | |
| dc.description.abstract | Plasmodium falciparum histidine-rich protein 2 (PfHRP 2) was selected in this work as the biomarker for the detection and diagnosis of malaria. An enzyme-linked immunosorbent assay (ELISA) was first developed to evaluate the immunoreagent’s suitability for the sensor’s development. A gold-based sensor with an integrated counter and an Ag/AgCl reference electrode was first selected and characterised and then used to develop the immunosensor for PfHRP 2, which enables a low cost, easy to use, and sensitive biosensor for malaria diagnosis. The sensor was applied to immobilise the anti-PfHRP 2 monoclonal antibody as the capture receptor. A sandwich ELISA assay format was constructed using horseradish peroxidase (HRP) as the enzyme label, and the electrochemical signal was generated using a 3, 3′, 5, 5′tetramethyl-benzidine dihydrochloride (TMB)/H2O2 system. The performance of the assay and the sensor were optimised and characterised, achieving a PfHRP 2 limit of detection (LOD) of 2.14 ng·mL−1 in buffer samples and 2.95 ng∙mL−1 in 100% spiked serum samples. The assay signal was then amplified using gold nanoparticles conjugated detection antibody-enzyme and a detection limit of 36 pg∙mL−1 was achieved in buffer samples and 40 pg∙mL−1 in serum samples. This sensor format is ideal for malaria detection and on-site analysis as a point-of-care device (POC) in resource-limited settings where the implementation of malaria diagnostics is essential in control and elimination efforts. | en_UK |
| dc.identifier.citation | Hemben A, Ashley J, Tothill IE, Development of an immunosensor for PfHRP 2 as a biomarker for Malaria detection, Biosensors, Vol. 7, Issue 3, 18 July 2017, article number 28, pp.1-14 | en_UK |
| dc.identifier.issn | 0265-928X | |
| dc.identifier.uri | http://dx.doi.org/10.3390/bios7030028 | |
| dc.identifier.uri | https://dspace.lib.cranfield.ac.uk/handle/1826/12217 | |
| dc.language.iso | en | en_UK |
| dc.publisher | MDPI | en_UK |
| dc.rights | Attribution 4.0 International (CC BY 4.0) You are free to: Share — copy and redistribute the material in any medium or format, Adapt — remix, transform, and build upon the material for any purpose, even commercially. The licensor cannot revoke these freedoms as long as you follow the license terms. Under the following terms: Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. Information: No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits. | |
| dc.subject | Malaria | en_UK |
| dc.subject | PfHRP 2 | en_UK |
| dc.subject | Parasites | en_UK |
| dc.subject | Immunosensor | en_UK |
| dc.subject | Biosensor | en_UK |
| dc.subject | Nanoparticles | en_UK |
| dc.title | Development of an immunosensor for PfHRP 2 as a biomarker for Malaria detection | en_UK |
| dc.type | Article | en_UK |