Molecular epidemiology and antimicrobial resistance of outbreaks of Klebsiella pneumoniae clinical mastitis in Chinese dairy farms

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dc.contributor.authorFu, Shaodong
dc.contributor.authorWen, Chen
dc.contributor.authorWang, Zhenglei
dc.contributor.authorQiu, Yawei
dc.contributor.authorZhang, Yihao
dc.contributor.authorZuo, Jiakun
dc.contributor.authorXu, Yuanyuan
dc.contributor.authorHan, Xiangan
dc.contributor.authorLuo, Zhenhua
dc.contributor.authorChen, Wei
dc.contributor.authorMiao, Jinfeng
dc.date.accessioned2022-12-12T13:38:31Z
dc.date.available2022-12-12T13:38:31Z
dc.date.issued2022-11-14
dc.description.abstractKlebsiella pneumoniae is an opportunistic pathogen that causes serious infections in humans and animals. However, the availability of epidemiological information on clinical mastitis due to K. pneumoniae is limited. To acquire new information regarding K. pneumoniae mastitis, data were mined about K. pneumoniae strains on dairy cattle farms (farms A to H) in 7 Chinese provinces in 2021. Hypermucoviscous strains of K. pneumoniae were obtained by the string test. MICs of antimicrobial agents were determined via the broth microdilution method. Ten antimicrobial resistance genes and virulence genes were identified by PCR. The prevalence of K. pneumoniae was 35.91% (65/181), and 100% of the bacteria were sensitive to enrofloxacin. Nine antimicrobial resistance genes and virulence genes were identified and compared among farms. The hypermucoviscous phenotype was present in 94.44% of isolates from farm B, which may be a function of the rmpA virulence gene. Based on these data, the multidrug-resistant strains SD-14 and HB-21 were chosen and sequenced. Genotypes were assayed for K. pneumoniae isolates from different countries and different hosts using multilocus sequence typing (MLST). Ninety-four sequence types (STs) were found, and 6 STs present a risk for spreading in specific regions. Interestingly, ST43 was observed in bovine isolates for the first time. Our study partially reveals the current distribution characteristics of bovine K. pneumoniae in China and may provide a theoretical basis for the prevention and treatment of bovine K. pneumoniae mastitis.en_UK
dc.identifier.citationFu S, Wen C, Wang Z, et al., (2022) Molecular epidemiology and antimicrobial resistance of outbreaks of Klebsiella pneumoniae clinical mastitis in Chinese dairy farms. Microbiology Spectrum, Volume 10, Number 6, December 2022, Article number e02997-22en_UK
dc.identifier.issn2165-0497
dc.identifier.urihttps://doi.org/10.1128/spectrum.02997-22
dc.identifier.urihttps://dspace.lib.cranfield.ac.uk/handle/1826/18782
dc.language.isoenen_UK
dc.publisherAmerican Society for Microbiologyen_UK
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectantimicrobial resistanceen_UK
dc.subjectbovine clinical mastitisen_UK
dc.subjectKlebsiella pneumoniaeen_UK
dc.subjectmolecular epidemiologyen_UK
dc.titleMolecular epidemiology and antimicrobial resistance of outbreaks of Klebsiella pneumoniae clinical mastitis in Chinese dairy farmsen_UK
dc.typeArticleen_UK

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