Water dynamics in Shewanella oneidensis at ambient and high pressure using quasi-elastic neutron scattering

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dc.contributor.authorFoglia, Fabrizia
dc.contributor.authorHazael, Rachael
dc.contributor.authorSimeoni, Giovanna G.
dc.contributor.authorAppavou, Marie-Sousai
dc.contributor.authorMoulin, Martine
dc.contributor.authorHaertlein, Michael
dc.contributor.authorForsyth, V. Trevor
dc.contributor.authorSeydel, Tilo
dc.contributor.authorDaniel, Isabelle
dc.contributor.authorMeersman, Filip
dc.contributor.authorMcMillan, Paul F.
dc.date.accessioned2018-02-16T15:35:45Z
dc.date.available2018-02-16T15:35:45Z
dc.date.issued2016-01-07
dc.description.abstractQuasielastic neutron scattering (QENS) is an ideal technique for studying water transport and relaxation dynamics at pico- to nanosecond timescales and at length scales relevant to cellular dimensions. Studies of high pressure dynamic effects in live organisms are needed to understand Earth’s deep biosphere and biotechnology applications. Here we applied QENS to study water transport in Shewanella oneidensis at ambient (0.1 MPa) and high (200 MPa) pressure using H/D isotopic contrast experiments for normal and perdeuterated bacteria and buffer solutions to distinguish intracellular and transmembrane processes. The results indicate that intracellular water dynamics are comparable with bulk diffusion rates in aqueous fluids at ambient conditions but a significant reduction occurs in high pressure mobility. We interpret this as due to enhanced interactions with macromolecules in the nanoconfined environment. Overall diffusion rates across the cell envelope also occur at similar rates but unexpected narrowing of the QENS signal appears between momentum transfer values Q = 0.7–1.1 Å−1 corresponding to real space dimensions of 6–9 Å. The relaxation time increase can be explained by correlated dynamics of molecules passing through Aquaporin water transport complexes located within the inner or outer membrane structures.en_UK
dc.identifier.citationFoglia F, Hazael R, Simeoni GG, Appavou M-S, Moulin M, Haertlein M, Forsyth VT, Seydel T, Daniel I, Meersman F, McMillan PF, Water dynamics in Shewanella oneidensis at ambient and high pressure using quasi-elastic neutron scattering, Scientific Reports, Vol. 6, 2016, Article number 18862en_UK
dc.identifier.cris19469733
dc.identifier.issn2045-2322
dc.identifier.urihttp://dx.doi.org/10.1038/srep18862
dc.identifier.urihttp://dspace.lib.cranfield.ac.uk/handle/1826/13008
dc.language.isoenen_UK
dc.publisherNature Publishing Groupen_UK
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleWater dynamics in Shewanella oneidensis at ambient and high pressure using quasi-elastic neutron scatteringen_UK
dc.typeArticleen_UK

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