Impedimetric biosensors for the quantification of serum biomarkers for early detection of lung cancer

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dc.contributor.authorArabnejad, Mahdi
dc.contributor.authorTothill, Ibtisam E.
dc.contributor.authorChianella, Iva
dc.date.accessioned2025-01-14T16:13:08Z
dc.date.available2025-01-14T16:13:08Z
dc.date.freetoread2025-01-14
dc.date.issued2024-12-01
dc.date.pubOnline2024-12-18
dc.descriptionData supporting this study are openly available from Cranfield University repository, CERES, at: https://dspace.lib.cranfield.ac.uk/handle/1826/19632, accessed on 16 November 2024.
dc.description.abstractLung cancer is the most common type of cancer diagnosed worldwide and is also among the most fatal. Early detection, before symptoms become evident, is fundamental for patients’ survival. Therefore, several lung cancer biomarkers have been proposed to enable a prompt diagnosis, including neuron-specific enolase (NSE) and carcinoembryonic antigen (CEA). NSE and CEA are two serum proteins whose elevated levels have been associated with lung cancer. Hence, in this study, impedimetric biosensors (immunosensors) able to quantify NSE and CEA were developed as proof-of-concept devices for lung cancer diagnosis. The sensing platform exploited for the immunosensors comprises a novel combination of a magnetic platform, screen-printed gold electrode (SPGE), and magnetic nanobeads (MB). The MB were functionalized with antibodies to capture the analyte from the sample and to move it over the sensing area. The immunosensors were then developed by immobilizing another set of antibodies for either CEA or NSE on the SPGE through formation of self-assembled monolayer (SAM). The second set of antibodies enabled a sandwich assay to be formed on the surface of the sensor, while MB manipulation was applied during the sensor performance to depict a microfluidic system and increase antigen–antibody complex formation prior to CEA or NSE detection and quantification. The optimized immunosensors were successfully tested to measure various concentrations of CEA and NSE (0–100 ng/mL) in both phosphate buffer and 100% human serum samples. Clinically relevant detection limits of 0.26 ng/mL and 0.18 ng/mL in buffer and 0.76 ng/mL and 0.52 ng/mL in 100% serum for CEA and NSE, respectively, were achieved via electrochemical impedance spectroscopy with the use of potassium ferri/ferrocyanide as a redox probe. Hence, the two immunosensors demonstrated great potential as tools to be implemented for the early detection of lung cancer.
dc.description.journalNameBiosensors
dc.format.mediumElectronic
dc.identifier.citationArabnejad M, Tothill IE, Chianella I. (2024) Impedimetric biosensors for the quantification of serum biomarkers for early detection of lung cancer. Biosensors, Volume 14, Issue 12, December 2024, Article number 624
dc.identifier.eissn2079-6374
dc.identifier.elementsID561010
dc.identifier.issn2079-6374
dc.identifier.issueNo12
dc.identifier.paperNo624
dc.identifier.urihttps://doi.org/10.3390/bios14120624
dc.identifier.urihttps://dspace.lib.cranfield.ac.uk/handle/1826/23380
dc.identifier.volumeNo14
dc.languageEnglish
dc.language.isoen
dc.publisherMDPI
dc.publisher.urihttps://www.mdpi.com/2079-6374/14/12/624
dc.relation.isreferencedbyhttps://dspace.lib.cranfield.ac.uk/handle/1826/19632
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectelectrochemical impedance spectroscopy
dc.subjectneuron-specific enolase
dc.subjectcarcinoembryonic antigen
dc.subjectimmunosensors
dc.subjectmagnetic nanobeads
dc.subject3401 Analytical Chemistry
dc.subject34 Chemical Sciences
dc.subjectLung Cancer
dc.subjectCancer
dc.subjectBiotechnology
dc.subjectLung
dc.subjectBioengineering
dc.subjectPrevention
dc.subject4.1 Discovery and preclinical testing of markers and technologies
dc.subjectCancer
dc.subjectcarcinoembryonic antigen
dc.subjectelectrochemical impedance spectroscopy
dc.subjectimmunosensors
dc.subjectmagnetic nanobeads
dc.subjectneuron-specific enolase
dc.subject3101 Biochemistry and cell biology
dc.subject3401 Analytical chemistry
dc.subject.meshHumans
dc.subject.meshLung Neoplasms
dc.subject.meshGold
dc.subject.meshPhosphopyruvate Hydratase
dc.subject.meshCarcinoembryonic Antigen
dc.subject.meshImmunoassay
dc.subject.meshBiosensing Techniques
dc.subject.meshElectrodes
dc.subject.meshEarly Detection of Cancer
dc.subject.meshBiomarkers, Tumor
dc.subject.meshBiosensing Techniques
dc.subject.meshHumans
dc.subject.meshLung Neoplasms
dc.subject.meshCarcinoembryonic Antigen
dc.subject.meshBiomarkers, Tumor
dc.subject.meshPhosphopyruvate Hydratase
dc.subject.meshEarly Detection of Cancer
dc.subject.meshGold
dc.subject.meshElectrodes
dc.subject.meshImmunoassay
dc.titleImpedimetric biosensors for the quantification of serum biomarkers for early detection of lung cancer
dc.typeArticle
dc.type.subtypeJournal Article
dcterms.coverageSwitzerland
dcterms.dateAccepted2024-12-14

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