A DNA-barcode biodiversity standard analysis method (DNA-BSAM) reveals a large variance in the effect of a range of biological, chemical and physical soil management interventions at different sites, but location is one of the most important aspects determining the nature of agricultural soil microbiology

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dc.contributor.authorFernández-Huarte, Matías
dc.contributor.authorElphinstone, John G.
dc.contributor.authorAdams, Ian P.
dc.contributor.authorVicente, Joana G.
dc.contributor.authorBhogal, Anne
dc.contributor.authorWatson, Christine A.
dc.contributor.authorDussart, Francois
dc.contributor.authorStockdale, Elizabeth A.
dc.contributor.authorWalshaw, John
dc.contributor.authorMcGreig, Sam
dc.contributor.authorSimmons, Robert W.
dc.contributor.authorMašková, Lucie
dc.contributor.authorDeeks, Lynda K.
dc.contributor.authorGoddard, Matthew R.
dc.date.accessioned2023-07-12T11:47:21Z
dc.date.available2023-07-12T11:47:21Z
dc.date.issued2023-07-06
dc.description.abstractThere are significant knowledge gaps in our understanding of how to sustainably manage agricultural soils to preserve soil biodiversity. Here we evaluate and quantify the effects of agricultural management and location on soil microbiology using nine field trials that have consistently applied different soil management practices in the United Kingdom using DNA barcode sequence data. We tested the basic hypothesis that various agricultural management interventions have a significant and greater effect on soil bacterial and fungal diversity than geographic location. The analyses of soil microbial DNA sequence data to date has lacked standardisation which prevents meaningful comparisons across sites and studies. Therefore, to analyse these data and crucially compare and quantify the size of any effects on soil bacterial and fungal biodiversity between sites, we developed and employed a post-sequencing DNA-barcode biodiversity standard analysis method (DNA-BSAM). The DNA-BSAM comprises a series of standardised bioinformatic steps for processing sequences but more importantly defines a standardised set of ecological indices and statistical tests. Use of the DNA-BSAM reveals the hypothesis was not strongly supported, and this was primarily because: 1) there was a large variance in the effects of various management interventions at different sites, and 2) that location had an equivalent or greater effect size than most management interventions for most metrics. Some dispersed sites imposed the same organic amendments interventions but showed different responses, and this combined with observations of strong differences in soil microbiomes by location tentatively suggests that any effect of management may be contingent on location. This means it could be unreliable to extrapolate the findings of individual trials to others. The widespread use of a standard approach will allow meaningful cross-comparisons between soil microbiome studies and thus a substantial evidence-base of the effects of land-use on soil microbiology to accumulate and inform soil management decisions.en_UK
dc.description.sponsorshipAgriculture and Horticulture Development Board (AHDB); British Beet Research Organisation (BBRO)en_UK
dc.identifier.citationFernández-Huarte M, Elphinstone JG, Adams IP, et al., (2023) A DNA-barcode biodiversity standard analysis method (DNA-BSAM) reveals a large variance in the effect of a range of biological, chemical and physical soil management interventions at different sites, but location is one of the most important aspects determining the nature of agricultural soil microbiology, Soil Biology and Biochemistry, Volume 184, September 2023, Article Number 109104en_UK
dc.identifier.eissn1879-3428
dc.identifier.issn0038-0717
dc.identifier.urihttps://doi.org/10.1016/j.soilbio.2023.109104
dc.identifier.urihttps://dspace.lib.cranfield.ac.uk/handle/1826/19966
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectMetabarcodingen_UK
dc.subjectAmplicon sequencingen_UK
dc.subjectSoil microbiologyen_UK
dc.subjectBiodiversityen_UK
dc.subjectSustainable soil managementen_UK
dc.subject16Sen_UK
dc.subjectITSen_UK
dc.titleA DNA-barcode biodiversity standard analysis method (DNA-BSAM) reveals a large variance in the effect of a range of biological, chemical and physical soil management interventions at different sites, but location is one of the most important aspects determining the nature of agricultural soil microbiologyen_UK

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