The mechanism of cryptolepine-induced cell death
| dc.contributor.author | Ansah, C. | - |
| dc.contributor.author | Zhu, H. | - |
| dc.contributor.author | Goderham, N. J. | - |
| dc.date.accessioned | 2012-07-03T23:00:59Z | |
| dc.date.available | 2012-07-03T23:00:59Z | |
| dc.date.issued | 2008-12-31T00:00:00Z | - |
| dc.description.abstract | The objective of the present study was to use morphological and biochemical approaches to characterize the mode of CLP-induced cell death. Using a differential staining technique, a Chinese Hamster fibroblast cell line (V79 cells) and a human lymphoblastoid cell line (MCL-5) showed morphology consistent with apoptosis after treatment with CLP. In contrast, HepG2, a human hepatoma cell line showed morphology that was more like necrosis after treatment with CLP. Using annexin V staining for apoptotic cells, MCL-5 cells showed a three fold increase in apoptosis within 6 h. Although we observed only a marginal increase in BAX protein expression, cytochrome c was released into the cytosol of CLP-treated MCL-5 cells. Furthermore, procaspase-3 was processed into the active caspase-3 (17 kDa). Consistent with the caspase-3 activation, PARP was cleaved to the typical 85 kDa fragment confirming apoptosis as the mode of cell death in CLP-treated MCL-5 cells. However, there was no evidence of increased BAX expression, cytochrome c release, caspase activation or PARP cleavage in CLP-treated HepG2 cells. This observation together with the morphology of CLP- treated HepG2 cells indicates that in contrast to MCL-5 cells, the CLP-mediated demise of HepG2 cells is not apoptotic. | en_UK |
| dc.identifier.citation | C. Ansah, H. Zhu, N. J. Goderham, The mechanism of ryptolepine-induced cell death. Journal of Pharmacology and Toxicology, 2008, Volume 3, Issue 4, pp291-301 | |
| dc.identifier.issn | 1816-496X | - |
| dc.identifier.uri | http://dx.doi.org/10.3923/jpt.2008.291.301 | - |
| dc.identifier.uri | http://dspace.lib.cranfield.ac.uk/handle/1826/7333 | |
| dc.publisher | Asian Network for Scientific Information | en_UK |
| dc.title | The mechanism of cryptolepine-induced cell death | en_UK |
| dc.type | Article | - |