Dissemination of metaldehyde catabolic pathways is driven by mobile genetic elements in Proteobacteria

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dc.contributor.authorCastro-Gutierrez, Víctor
dc.contributor.authorFuller, Edward
dc.contributor.authorGarcillán-Barcia, María Pilar
dc.contributor.authorHelgason, Thorunn
dc.contributor.authorHassard, Francis
dc.contributor.authorMoir, James
dc.date.accessioned2022-11-15T10:51:47Z
dc.date.available2022-11-15T10:51:47Z
dc.date.issued2022-10-27
dc.description.abstractBioremediation of metaldehyde from drinking water using metaldehyde-degrading strains has recently emerged as a promising alternative. Whole-genome sequencing was used to obtain full genomes for metaldehyde degraders Acinetobacter calcoaceticus E1 and Sphingobium CMET-H. For the former, the genetic context of the metaldehyde-degrading genes had not been explored, while for the latter, none of the degrading genes themselves had been identified. In A. calcoaceticus E1, IS91 and IS6-family insertion sequences (ISs) were found surrounding the metaldehyde-degrading gene cluster located in plasmid pAME76. This cluster was located in closely-related plasmids and associated to identical ISs in most metaldehyde-degrading β- and γ-Proteobacteria, indicating horizontal gene transfer (HGT). For Sphingobium CMET-H, sequence analysis suggested a phytanoyl-CoA family oxygenase as a metaldehyde-degrading gene candidate due to its close homology to a previously identified metaldehyde-degrading gene known as mahX. Heterologous gene expression in Escherichia coli alongside degradation tests verified its functional significance and the degrading gene homolog was henceforth called mahS. It was found that mahS is hosted within the conjugative plasmid pSM1 and its genetic context suggested a crossover between the metaldehyde and acetoin degradation pathways. Here, specific replicons and ISs responsible for maintaining and dispersing metaldehyde-degrading genes in α, β and γ-Proteobacteria through HGT were identified and described. In addition, a homologous gene implicated in the first step of metaldehyde utilisation in an α-Proteobacteria was uncovered. Insights into specific steps of this possible degradation pathway are provided.en_UK
dc.identifier.citationCastro-Gutierrez V, Fuller E, Garcillán-Barcia MP, et al., (2022) Dissemination of metaldehyde catabolic pathways is driven by mobile genetic elements in Proteobacteria. Microbial Genomics, Volume 8, Issue 10, October 2022, Article number 000881en_UK
dc.identifier.issn2057-5858
dc.identifier.urihttps://doi.org/10.1099/mgen.0.000881
dc.identifier.urihttps://dspace.lib.cranfield.ac.uk/handle/1826/18711
dc.language.isoenen_UK
dc.publisherThe Microbiology Societyen_UK
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectbioremediationen_UK
dc.subjectevolutionen_UK
dc.subjectinsertion sequenceen_UK
dc.subjectmetaldehydeen_UK
dc.subjectpesticidesen_UK
dc.subjectplasmiden_UK
dc.titleDissemination of metaldehyde catabolic pathways is driven by mobile genetic elements in Proteobacteriaen_UK
dc.typeArticleen_UK

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