Sampling microbial volatile organic compounds: optimisation of flow rate and sampling time

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dc.contributor.authorGarcia Alcega, Sonia
dc.contributor.authorTyrrel, Sean
dc.contributor.authorCoulon, Frederic
dc.date.accessioned2017-09-21T13:17:49Z
dc.date.available2017-09-21T13:17:49Z
dc.date.issued2017-08-06
dc.description.abstractThe impact of bioaerosols emissions from urban, agricultural and industrial environments on local air quality is of growing policy concern. However, there is no standardised protocol established yet, despite a large number of bioaerosols sampling methods in use. Additionally, capturing sufficient amounts of material to allow reproducible separation and detection of molecular patterns is still difficult. Chemical fingerprint analysis of microbial volatile organic compounds (MVOC) is a potentially rapid and reproducible approach for the early detection and identification of outdoor contamination as it has been shown to be a successful approach for indoor environments and it can be done on a fine-scale, allowing the identification of species-specific volatiles that may serve as marker compounds for the selective detection of pathogens. In this study we have tested the number and concentration of MVOCs collected using different sampling conditions: 10 min sampling time with variable flow rate (100, 500 and 1000 ml min–1) and 100 ml min–1 flow rate during 10, 20 and 30 min using Tenax®-Carbotrap thermal desorption (TD) tubes attached to portable GilAir® air pumps. Our aim was to determine the best sampling conditions in order to get enough material allowing reproducible data of the microbial markers present in outdoor environments. Substantial loses (>50%) of MVOCs occurred when sampling at flow rates higher than 100 ml min–1. 10 min sampling time allowed the collection of most of the MVOCs present in the air (~96%). The optimal sampling settings that allowed the collection of higher concentrations of MVOCs without breakthrough was 10 min sampling at 100 ml min–1 flow rate. Ketones were the predominant group of MVOCs identified in the WWTP (34–42%), acetone being the compound present at higher concentration (6476–11731 ng m–3).en_UK
dc.identifier.citationGarcia Alcega S, Tyrrel S, Coulon F, Sampling microbial volatile organic compounds: optimisation of flow rate and sampling time, Air Pollution XXV: 25th International Conference on Modelling, Monitoring and Management of Air Pollution, 25-27 April, 2017, Cadiz, Spain, pp. 69-76en_UK
dc.identifier.issn1743-3541
dc.identifier.urihttp://dx.doi.org/10.2495/AIR170071
dc.identifier.urihttp://dspace.lib.cranfield.ac.uk/handle/1826/12531
dc.language.isoenen_UK
dc.publisherWIT Pressen_UK
dc.rightsAttribution 4.0 International (CC BY 4.0) You are free to: Share — copy and redistribute the material in any medium or format Adapt — remix, transform, and build upon the material for any purpose, even commercially. The licensor cannot revoke these freedoms as long as you follow the license terms. Under the following terms: Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. Information: No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
dc.subjectMVOCsen_UK
dc.subjectBioaerosolsen_UK
dc.subjectThermal desorptionen_UK
dc.subjectChemometricsen_UK
dc.subjectOutdoor environmentsen_UK
dc.titleSampling microbial volatile organic compounds: optimisation of flow rate and sampling timeen_UK
dc.typeConference paperen_UK

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