Novel highly sensitive protein sensors based on tapered optical fibres modified with Au-based nanocoatings

Simple item page
dc.contributor.authorUrrutia, Aitor
dc.contributor.authorBojan, Kartheka
dc.contributor.authorMarques, L.
dc.contributor.authorMullaney, Kevin
dc.contributor.authorGoicoechea, Javier
dc.contributor.authorJames, Stephen W.
dc.contributor.authorClark, M.
dc.contributor.authorTatam, Ralph P.
dc.contributor.authorKorposh, Sergiy
dc.date.accessioned2016-09-13T11:40:45Z
dc.date.available2016-09-13T11:40:45Z
dc.date.issued2016-08-08
dc.description.abstractIn this work, novel protein sensors based on tapered optical fibres modified with Au coatings deposited using two different procedures are proposed. Au based coatings are deposited onto a non-adiabatic tapered optical fibre using: (i) a novel facile method composed of layer-by-layer deposition consisting of polycation (poly(allylamine hydrochloride), PAH) and negatively charged SiO2 nanoparticles (NPs) followed by the deposition of the charged Au NPs; and (ii) the sputtering technique. The Au NPs and Au thin film surfaces are then modified with biotin in order to bind Streptavidin (SV) molecules and detect them. The sensing principle is based on the sensitivity of the transmission spectrum of the device to changes in the refractive index of the coatings induced by the SV binding to the biotin. Both sensors, showed high sensitivity to SV, with the lowest measured concentration levels below 2.5 nM. The calculated binding constant for the biotin-SV pair was 2.2 x10-11 M-1 when a tapered fibre modified with the LbL method was used, with a Limit of Detection (LoD) of 271 pM. The sensor formed using sputtering had a binding constant of 1.01x10-10 M-1 with a LoD of 806 pM. These new structures and their simple fabrication technique could be used to develop other bio-sensors.en_UK
dc.identifier.citationUrrutia, A. et al. (2016) Novel highly sensitive protein sensors based on tapered optical fibres modified with Au-based nanocoatings, Journal of Sensors, 2016, Article ID 8129387en_UK
dc.identifier.issn1687-725X
dc.identifier.urihttp://dx.doi.org/10.1155/2016/8129387
dc.identifier.urihttps://dspace.lib.cranfield.ac.uk/handle/1826/10525
dc.language.isoenen_UK
dc.publisherHindawi Publishing Corporationen_UK
dc.rightsAttribution 4.0 Internationalen_UK
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleNovel highly sensitive protein sensors based on tapered optical fibres modified with Au-based nanocoatingsen_UK
dc.typeArticleen_UK

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Novel_highly_sensitive_protein_sensors_based_on_tapered_optical_fibres_modified_with_Au-based_nanocoatings_2016.pdf
Size:
2.47 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.79 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Staff publications (SATM)