Electrochemical Immunoassay for Free Prostate Specific Antigen (f-PSA) Using Magnetic Beads

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dc.contributor.authorSarkar, P.
dc.contributor.authorGhosh, D.
dc.contributor.authorBhattacharyay, D.
dc.contributor.authorSetford, S. J.
dc.contributor.authorTurner, Anthony P. F.
dc.date.accessioned2009-06-12T09:38:44Z
dc.date.available2009-06-12T09:38:44Z
dc.date.issued2008
dc.description.abstractProstate specific antigen (PSA) is a prominent marker for the prostate carcinoma. It is found in human blood in free (f-PSA) and complex forms. These two forms together are called total PSA (t-PSA). Estimation of both forms is essential to predict malignancy. In this study we report a unique and effective technique of electrochemical detection of f-PSA using magnetic beads on a three-electrode screen-printed sensor. A magnetic bead enzyme linked immunosorbent assay (ELISA) was performed in a cuvette. Following the immunoassay, magnetic beads were recovered by a magnetic concentrator and transferred on the working electrode of the 3-electrode assembly. The amperometric response, a measure of the amount of residual enzyme activity on the beads and hence the concentration of analyte in solution, was determined by addition of enzyme substrate. The device has a detection limit of <0.1 ng mL-1 f-PSA and a linear range of 0 to 1 ng mL-1 f-PSA.en_UK
dc.identifier.citationP. Sarkar , D. Ghosh , D. Bhattacharyay , S. J. Setford , A. P. F. Turner, Electrochemical Immunoassay for Free Prostate Specific Antigen (f-PSA) Using Magnetic Beads, Electroanalysis, 2008, Volume 20, Issue 13, Pages 1414 to 1426en_UK
dc.identifier.issn1521-4109'
dc.identifier.urihttp://dx.doi.org/10.1002/elan.200804194
dc.identifier.urihttp://hdl.handle.net/1826/3451
dc.language.isoenen_UK
dc.publisherWiley InterScienceen_UK
dc.subjectBioanalytical methodsen_UK
dc.subjectBioassaysen_UK
dc.subjectBiosensorsen_UK
dc.subjectImmunoassaysen_UK
dc.subjectELISAen_UK
dc.titleElectrochemical Immunoassay for Free Prostate Specific Antigen (f-PSA) Using Magnetic Beadsen_UK
dc.typePostprinten_UK

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