Functionally different PIN proteins control auxin flux during bulbil development in Agave tequilana

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dc.contributor.authorAbraham Juarez, Maria Jazmin
dc.contributor.authorCardenas, Rocio Hernandez
dc.contributor.authorSantoyo Villa, Jose Natzul
dc.contributor.authorO'Connor, Devin
dc.contributor.authorSluis, Aaron
dc.contributor.authorHake, Sarah
dc.contributor.authorOrdaz-Ortiz, José J.
dc.contributor.authorTerry, Leon A
dc.contributor.authorSimpson, June
dc.date.accessioned2016-09-28T09:07:50Z
dc.date.available2016-09-28T09:07:50Z
dc.date.issued2015-04-23
dc.description.abstractIn Agave tequilana, reproductive failure or inadequate flower development stimulates the formation of vegetative bulbils at the bracteoles, ensuring survival in a hostile environment. Little is known about the signals that trigger this probably unique phenomenon in agave species. Here we report that auxin plays a central role in bulbil development and show that the localization of PIN1-related proteins is consistent with altered auxin transport during this process. Analysis of agave transcriptome data led to the identification of the A. tequilana orthologue of PIN1 (denoted AtqPIN1) and a second closely related gene from a distinct clade reported as ‘Sister of PIN1’ (denoted AtqSoPIN1). Quantitative real-time reverse transcription–PCR (RT-qPCR) analysis showed different patterns of expression for each gene during bulbil formation, and heterologous expression of the A. tequilana PIN1 and SoPIN1 genes in Arabidopsis thaliana confirmed functional differences between these genes. Although no free auxin was detected in induced pedicel samples, changes in the levels of auxin precursors were observed. Taken as a whole, the data support the model that AtqPIN1 and AtqSoPIN1 have co-ordinated but distinct functions in relation to auxin transport during the initial stages of bulbil formation.en_UK
dc.identifier.citationAbraham Juárez et al. Journal of Experimental Botany 2015, Vol. 66, No. 13 pp. 3893–3905en_UK
dc.identifier.isbnhttp://jxb.oxfordjournals.org/content/66/13/3893
dc.identifier.issn0022-0957
dc.identifier.urihttp://dspace.lib.cranfield.ac.uk/handle/1826/10609
dc.language.isoenen_UK
dc.publisherOxford University Press (OUP)en_UK
dc.subjectagave tequilanaen_UK
dc.subjectauxin fluxen_UK
dc.subjectbulbilen_UK
dc.subjectdevelopmenten_UK
dc.subjectgene expressionen_UK
dc.subjectimmunolocalizationen_UK
dc.subjectPIN proteinsen_UK
dc.titleFunctionally different PIN proteins control auxin flux during bulbil development in Agave tequilanaen_UK
dc.typeArticleen_UK

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