Microfluidics for rapid detection of live pathogens

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dc.contributor.authorSpatola Rossi, Carla
dc.contributor.authorCoulon, Frederic
dc.contributor.authorMa, Shaohua
dc.contributor.authorZhang, Yu Shrike
dc.contributor.authorYang, Zhugen
dc.date.accessioned2023-03-22T15:01:18Z
dc.date.available2023-03-22T15:01:18Z
dc.date.issued2023-03-15
dc.description.abstractRapid, sensitive, and selective detection of live pathogens remains a key priority for quality control and risk assessment. While conventional methods often require complicated workflows, costly reagents, lab equipment, and are time-consuming, rendering them inadequate for field testing and low-resource settings. Increased attention has been drawn to developing alternative low-cost and rapid methods to detect on-site live pathogens in different environmental matrices. Among them, microfluidic devices that integrate various laboratory functions in a miniaturized manner have proven to be a promising tool for the rapid and sensitive detection of pathogens. Herein, the development of microfluidic devices specifically designed for the detection of live pathogens is discussed along a concise summary of novel microfluidics systems recently developed, contrasted to conventional methods regarding assay time, the limit of detection, and target organisms. These include a variety of micro total analysis systems (µTAS) and microfluidic paper-based analytical devices (µPADs) in combination with molecular methods and traditional live cell detection techniques, such as cell culture, DNA intercalating dyes, resazurin, and immobilized bioreceptors (e.g., aptamers and capture antibodies). Furthermore, insights on the future perspectives of microfluidics for live pathogen detection with a highlight on the rapid and low-cost method development for field testing are provided.en_UK
dc.identifier.citationSpatola Rossi C, Coulon F, Ma S, et al., (2023) Microfluidics for rapid detection of live pathogens, Advanced Functional Materials, Volume 33, Issue 21, May 17, 2023, Article Number 2212081en_UK
dc.identifier.issn1616-301X
dc.identifier.urihttps://doi.org/10.1002/adfm.202212081
dc.identifier.urihttps://dspace.lib.cranfield.ac.uk/handle/1826/19337
dc.language.isoenen_UK
dc.publisherWileyen_UK
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectMicrofluidicsen_UK
dc.subjectcell viabilityen_UK
dc.subjectpathogen detectionen_UK
dc.subjectsensorsen_UK
dc.titleMicrofluidics for rapid detection of live pathogensen_UK
dc.typeArticleen_UK

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