Piriformospora indica based elicitation for overproduction of phenolic compounds by hairy root cultures of Ficus carica

Simple item page
dc.contributor.authorAmani, Shahla
dc.contributor.authorMohebodini, Mehdi
dc.contributor.authorKhademvatan, Shahram
dc.contributor.authorJafari, Morad
dc.contributor.authorKumar, Vinod
dc.date.accessioned2021-01-20T16:13:52Z
dc.date.available2021-01-20T16:13:52Z
dc.date.issued2020-12-30
dc.description.abstractFicus carica L. is an important source of phenolic and flavonoid compounds with valuable pharmaceutical application across various diseases. The current study was carried out to investigate the influence of Piriformospora indica elicitation on growth, production of phenolic compounds, antioxidant capacity, and expression level of flavonoid biosynthetic pathway genes in hairy root (HR) cultures of F. carica. The maximum improvement in accumulation of phenolic compounds was observed when HR culture of Ficus carica L. was exposed to 2% culture filtrate of P. indica for 72 h: gallic acid (80.5- fold), caffeic acid (26.2-fold), coumaric acid (4.5-fold), and cinnamic acid (60.1-fold), apigenin (27.6-fold) and rutin (5.7-fold). While the highest levels of chlorogenic acid (4.9-fold) and quercetin flavonoid (8.8-fold) were obtained after 48 h elicitation with culture filtrate and cell extract of P. indica at 6% (v/v), respectively. The analysis of biosynthetic genes revealed that the exposure to fungal elicitors resulted in up-regulation of phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), UDP-glucose flavonoid 3-O-glucosyltransferase (UFGT) and MYB3 transcription factor. This study shows the potential of P. indica as an efficacious elicitor for enhancing the secondary metabolites production by F. carica HRs.en_UK
dc.identifier.citationAmani S, Mohebodini M, Khademvatan S, et al., (2021) Piriformospora indica based elicitation for overproduction of phenolic compounds by hairy root cultures of Ficus carica. Journal of Biotechnology, Volume 327, February 2021, pp. 43-53en_UK
dc.identifier.issn0168-1656
dc.identifier.urihttps://doi.org/10.1016/j.jbiotec.2020.12.015
dc.identifier.urihttp://dspace.lib.cranfield.ac.uk/handle/1826/16212
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectFicus carica L.en_UK
dc.subjectFungal elicitoren_UK
dc.subjectPhenolic compoundsen_UK
dc.subjectPiriformospora indicaen_UK
dc.subjectElicitationen_UK
dc.subjectGene expressionen_UK
dc.titlePiriformospora indica based elicitation for overproduction of phenolic compounds by hairy root cultures of Ficus caricaen_UK
dc.typeArticleen_UK

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Piriformospora_indica_based_elicitation-2020.pdf
Size:
771.07 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.63 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Staff publications (SWEE)