A multi-modal exploration of heterogeneous physico–chemical properties of DCIS breast microcalcifications

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dc.contributor.authorGosling, Sarah
dc.contributor.authorCalabrese, Doriana
dc.contributor.authorNallala, Jayakrupakar
dc.contributor.authorGreenwood, Charlene
dc.contributor.authorPinder, Sarah
dc.contributor.authorKing, Lorraine
dc.contributor.authorMarks, Jeffrey
dc.contributor.authorPinto, Donna
dc.contributor.authorLynch, Thomas
dc.contributor.authorLyburn, Iain Douglas
dc.contributor.authorHwang, Shelley
dc.contributor.authorGrand Challenge PRECISION Consortium
dc.contributor.authorRogers, Keith
dc.contributor.authorStone, Nicholas
dc.date.accessioned2022-03-31T08:14:26Z
dc.date.available2022-03-31T08:14:26Z
dc.date.issued2022-03-21
dc.description.abstractDuctal carcinoma in situ (DCIS) is frequently associated with breast calcification. This study combines multiple analytical techniques to investigate the heterogeneity of these calcifications at the micrometre scale. X-ray diffraction, scanning electron microscopy and Raman and Fourier-transform infrared spectroscopy were used to determine the physicochemical and crystallographic properties of type II breast calcifications located in formalin fixed paraffin embedded DCIS breast tissue samples. Multiple calcium phosphate phases were identified across the calcifications, distributed in different patterns. Hydroxyapatite was the dominant mineral, with magnesium whitlockite found at the calcification edge. Amorphous calcium phosphate and octacalcium phosphate were also identified close to the calcification edge at the apparent mineral/matrix barrier. Crystallographic features of hydroxyapatite also varied across the calcifications, with higher crystallinity centrally, and highest carbonate substitution at the calcification edge. Protein was also differentially distributed across the calcification and the surrounding soft tissue, with collagen and β-pleated protein features present to differing extents. Combination of analytical techniques in this study was essential to understand the heterogeneity of breast calcifications and how this may link crystallographic and physicochemical properties of calcifications to the surrounding tissue microenvironment.en_UK
dc.description.sponsorshipCancer Research UK and by KWF Kankerbestrijding: C38317/A24043en_UK
dc.identifier.citationGosling S, Calabrese D, Nallala J, et al., (2022) A multi-modal exploration of heterogeneous physico–chemical properties of DCIS breast microcalcifications, The Analyst, Volume 147, Issue 8, 21 April 2022, pp. 1641-1654en_UK
dc.identifier.eissn1364-5528
dc.identifier.issn0003-2654
dc.identifier.urihttps://doi.org/10.1039/D1AN01548F
dc.identifier.urihttp://dspace.lib.cranfield.ac.uk/handle/1826/17720
dc.language.isoenen_UK
dc.publisherRoyal Society of Chemistryen_UK
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleA multi-modal exploration of heterogeneous physico–chemical properties of DCIS breast microcalcificationsen_UK
dc.typeArticleen_UK

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