Biological production and recovery of 2,3-butanediol using arabinose from sugar beet pulp by Enterobacter ludwigii

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dc.contributor.authorNarisetty, Vivek
dc.contributor.authorNarisetty, Sudheera
dc.contributor.authorJacob, Samuel
dc.contributor.authorKumar, Deepak
dc.contributor.authorLeeke, Gary A.
dc.contributor.authorChandel, Anuj Kumar
dc.contributor.authorSingh, Vijai
dc.contributor.authorSrivastava, Vimal Chandra
dc.contributor.authorKumar, Vinod
dc.date.accessioned2022-06-17T08:00:09Z
dc.date.available2022-06-17T08:00:09Z
dc.date.issued2022-04-21
dc.description.abstractSugar beet pulp (SBP) is a major byproduct from the sugar industries and consists of >20% w/w arabinose. The current work evaluated the potential of Enterobacter ludwigii assimilating pure arabinose and arabinose rich hydrolysate from SBP pellets for 2,3-butanediol (BDO) production. The hydrolysate was obtained through dilute acid pretreatment (DAP) with sulphuric acid. The process was optimized for acid and solid loading to obtain a hydrolysate free from furan derivatives. The effect of different levels of substrate (10–60 g/L) using pure arabinose was conducted in shake flask experiments, followed by co-fermentation with small amounts of glucose and SBP hydrolysate. After flask cultivations, BDO fermentations were carried-out in a bench-top bioreactor in batch and fed-batch modes using pure arabinose as well as SBP hydrolysate. The fed-batch culture led to BDO production of 42.9 and 35.5 g/L from pure arabinose and SBP hydrolysate with conversion yields of 0.31 and 0.29 g/g, respectively. Finally, BDO accumulated on pure arabinose and SBP hydrolysate were recovered using an aqueous two-phase extraction system. The recovery yield of BDO accumulated on arabinose and hydrolysate was ∼97%. The work demonstrated the feasibility of using SBP as a suitable feedstock for manufacturing BDO.en_UK
dc.identifier.citationNarisetty V, Narisetty S, Jacob S, et al., (2022) Biological production and recovery of 2,3-butanediol using arabinose from sugar beet pulp by Enterobacter ludwigii. Renewable Energy, Volume 191, May 2022, pp. 394-404en_UK
dc.identifier.issn0960-1481
dc.identifier.urihttps://doi.org/10.1016/j.renene.2022.04.024
dc.identifier.urihttp://dspace.lib.cranfield.ac.uk/handle/1826/18037
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject2,3-Butanediol; Arabinoseen_UK
dc.subjectSugar beet pulpen_UK
dc.subjectPretreatmenten_UK
dc.subjectAqueous two-phase extraction systemen_UK
dc.titleBiological production and recovery of 2,3-butanediol using arabinose from sugar beet pulp by Enterobacter ludwigiien_UK
dc.typeArticleen_UK

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