Production of extracellular enzymes by different isolates of Pochonia chlamydosporia

Simple item page
dc.contributor.authorEsteves, Ivania
dc.contributor.authorPeteira, Belkis
dc.contributor.authorAtkins, Simon D.
dc.contributor.authorMagan, Naresh
dc.contributor.authorKerry, Brian R.
dc.date.accessioned2009-08-08T14:15:11Z
dc.date.available2009-08-08T14:15:11Z
dc.date.issued2009-08
dc.description.abstractFor the first time, the specific activities of chitinases, esterases, lipases and a serine protease (VCP1) produced by different isolates of the nematophagous fungus Pochonia chlamydosporia were quantified and compared. The isolates were grown for different time periods in a minimal liquid medium or media supplemented with 1 % chitin, 0.2 % gelatin or 2 % olive oil. Enzyme-specific activities were quantified in filtered culture supernatants using chromogenic p-nitrophenyl substrates (for chitinases, lipases and esterases) and a p-nitroanilide substrate (to measure the activity of the proteinase VCP1). Additionally, information on parasitic growth (nematode egg parasitism) and saprotrophic growth (plant rhizosphere colonisation) was collected. Results showed that the production of extracellular enzymes was influenced by the type of medium (p < 0.05) in which P. chlamydosporia was grown. Enzyme activity differed with time (p < 0.05), and significant differences were found between isolates (p < 0.001) and the amounts of enzymes produced (p < 0.001). However, no significant relationships were found between enzyme activities and parasitic or saprotrophic growth using Kendall's coefficient of concordance or Spearman rank correlation coefficient. The results provided new information about enzyme production in P. chlamydosporia and suggested that the mechanisms which regulate the trophic switch in this fungus are complex and dependent on several factors.en_UK
dc.identifier.citationIvania Esteves, Belkis Peteira, Simon D. Atkins, Naresh Magan, Brian Kerry, Production of extracellular enzymes by different isolates of Pochonia chlamydosporia, Mycological Research, Volume 113, Issue 8, August 2009, Pages 867-876en_UK
dc.identifier.issn0953-7562
dc.identifier.urihttp://hdl.handle.net/1826/3562
dc.identifier.urihttp://dx.doi.org/10.1016/j.mycres.2009.04.005
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.subjectChitinasesen_UK
dc.subjectEnzyme activityen_UK
dc.subjectEsterasesen_UK
dc.subjectKendall's coefficient of concordanceen_UK
dc.subjectLipasesen_UK
dc.subjectp-nitrophenyl substratesen_UK
dc.subjectPochonia chlamydosporiaen_UK
dc.subjectProteasesen_UK
dc.subjectSpearman rank correlation coefficienten_UK
dc.titleProduction of extracellular enzymes by different isolates of Pochonia chlamydosporiaen_UK
dc.typeArticleen_UK

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Production_of_extracellular_enzymes_by_different_isolates_of_Pochonia_chlamydosporia-2009.pdf
Size:
369.14 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.88 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Staff publications - Cranfield Health