Development of a β-Lactoglobulin sensor based on SPR for milk allergens detection
| dc.contributor.author | Ashley, Jon | |
| dc.contributor.author | D’Aurelio, Roberta | |
| dc.contributor.author | Piekarska, Monika | |
| dc.contributor.author | Temblay, Jeff | |
| dc.contributor.author | Pleasants, Mike | |
| dc.contributor.author | Trinh, Linda | |
| dc.contributor.author | Rodgers, Thomas L. | |
| dc.contributor.author | Tothill, Ibtisam E. | |
| dc.date.accessioned | 2019-01-09T15:54:25Z | |
| dc.date.available | 2019-01-09T15:54:25Z | |
| dc.date.issued | 2018-03-27 | |
| dc.description.abstract | A sensitive and label-free surface plasmon resonance (SPR) based sensor was developed in this work for the detection of milk allergens. β-lactoglobulin (BLG) protein was used as the biomarker for cow milk detection. This is to be used directly in final rinse samples of cleaning in-place (CIP) systems of food manufacturers. The affinity assay was optimised and characterised before a standard curve was performed in pure buffer conditions, giving a detection limit of 0.164 µg mL−1 as a direct binding assay. The detection limit can be further enhanced through the use of a sandwich assay and amplification with nanomaterials. However, this was not required here, as the detection limit achieved exceeded the required allergen detection levels of 2 µg mL−1 for β-lactoglobulin. The binding affinities of the polyclonal antibody for BLG, expressed by the dissociation constant (KD), were equal to 2.59 × 10−9 M. The developed SPR-based sensor offers several advantages in terms of label-free detection, real-time measurements, potential on-line system and superior sensitivity when compared to ELISA-based techniques. The method is novel for this application and could be applied to wider food allergen risk management decision(s) in food manufacturing. | en_UK |
| dc.identifier.citation | Jon Ashley, Roberta D’Aurelio, Monika Piekarska, et al., Development of a β-Lactoglobulin sensor based on SPR for milk allergens detection. Biosensors, 2018, Volume 8, Issue 2, Article number 32 | en_UK |
| dc.identifier.issn | 2079-6374 | |
| dc.identifier.uri | https://doi.org/10.3390/bios8020032 | |
| dc.identifier.uri | https://dspace.lib.cranfield.ac.uk/handle/1826/13810 | |
| dc.language.iso | en | en_UK |
| dc.publisher | MDPI | en_UK |
| dc.rights | Attribution 4.0 International | * |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
| dc.subject | Milk protein | en_UK |
| dc.subject | Surface plasmon resonance (SPR) | en_UK |
| dc.subject | Allergen | en_UK |
| dc.subject | Biosensor | en_UK |
| dc.subject | β-lactoglobulin (BLG) | en_UK |
| dc.title | Development of a β-Lactoglobulin sensor based on SPR for milk allergens detection | en_UK |
| dc.type | Article | en_UK |